After microCT scanning, the calvaria were dehydrated, embedded in Techovit® (Electron Microscopy Sciences, Hatfield, PA USA), and grinded 3 sections to expose a mid-sagittal view of the calvaria in 60 µm thickness using Exakt grinding instrument (Hamburg-Norderstedt, Germany). Stevenel’s blue and Van Gieson staining were performed to assess new bone formation. Color images of the histologic sections were acquired under 10x magnification using a Nikon Eclipse Ti-U camera with an automatic stage (Melville, NY USA) from three independent samples, and image analysis was performed by measuring in pixels using Image J software (U.S. National Institutes of Health, Bethesda, MD USA). The newly formed bone area was calculated as a percent of total defect area.
Eclipse ti u camera
Manufactured by Nikon
Sourced in United States
The Eclipse Ti-U camera is a high-performance microscope camera designed for advanced imaging applications. It features a large sensor, high resolution, and low noise performance, making it suitable for a variety of microscopy techniques.
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Lab products found in correlation
3 protocols using eclipse ti u camera
1
Histological Assessment of Bone Formation
2
Quantitative Analysis of Newly Formed Bone
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Calvaria explants from each group were stained using Stevenel’s Blue and counterstained with Van Gieson to visualize newly formed bone (NFB) tissue. Colored microscopic images of the medial (central) sagittal histologic sections were acquired under 10x magnification using Nikon Eclipse Ti-U camera with automatic stage, then merged using Nikon software to stich each image as one figure. The newly formed bone area (B.Ar.) and the total defect area (T.Ar.) were quantified using Image J software to calculate the NFB in % followed by the standardized protocol30 (link).
3
Fluorescence and Bright-field Microscopy
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Microscopy images were obtained of the samples with a wide-field fluorescence
confocal microscope; bright-field images were obtained using a Nikon Eclipse
Ti-U camera.
confocal microscope; bright-field images were obtained using a Nikon Eclipse
Ti-U camera.
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