Malic acid,
Diosgenin, Pluronic F68, 1, 12-Dodecanedioic acid (DDA), Pluronic 127 were purchased from Sigma Aldrich.
Acetone was obtained from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India).
Perchloric acid was purchased from Central Drug House (CDH) (Mumbai, India). For animal cell culture studies,
Phosphate buffered saline,
Alamar Blue and
DAPI or 4′,6-diamidino-2-phenylindole, sodium bicarbonate were procured from HiMedia (Mumbai, India).
Dulbecco’s modified Eagle’s medium (DMEM), Fetal Bovine Serum (FBS), Trypsin-EDTA (0.25%) were purchased from Gibco Life Technologies (Carlsbad, CA, USA). MilliQ water was used in the preparation of solutions during the study. A549, human adenocarcinoma cell lines were purchased from NCCS, Pune, India.
Sharma N., Singhal M., Kumari R.M., Gupta N., Manchanda R., Syed A., Bahkali A.H, & Nimesh S. (2020). Diosgenin Loaded Polymeric Nanoparticles with Potential Anticancer Efficacy. Biomolecules, 10(12), 1679.