Sod detection kit

The cellular ROS production was analyzed using 7′-Dichlorodihydrofluorescein Diacetate (DCFH-DA) staining (Jiancheng, China) according to the manufacturer's instruction. The NO levels were analyzed using the NO detection Kit (Solarbio, China). The SOD activities were analyzed using the SOD detection Kit (Solarbio, China).

The concentrations of superoxide anion and H₂O₂ were measured using detection assay kits (Solarbio, Beijing, China) following the manufacturer’s instructions. The ATP content was measured using phosphomolybdic acid colorimetry according to the ATP assay kit (G0815W96) purchased from Suzhou Grace Biotechnology Co. (Suzhou, China). For the enzyme assays, superoxide dismutase (SOD) activity was measured using a SOD detection kit (Solarbio, Beijing, China), and catalase (CAT) activity was measured following the instruction of CAT detection kit (Solarbio, Beijing, China), and peroxidase (POD) activity was detected with a POD detection kit (Solarbio, Beijing, China). T-AOC, mitochondrial complex I and V activities were measured by T-AOC assay kit, complex I assay kit and complex V (Solarbio, Beijing, China).