Mir 103a 3p mimics

miR-103a-3p mimics (5′-AGCAGCATTGTACAGGGCTATGA-3′), mimics negative control (NC) (5′-ATAGTGATCAGATGGGCAGCCTA-3′), miR-103a-3p inhibitor (5′-TCATAGCCCTGTACAATGCTGCT-3′), inhibitor NC (5′-TATCCCACGCTAGCTGTCTTGAA-3′) were obtained from Shanghai GenePharma Co., Ltd. pcDNA3.1-FBXW7, pcDNA3.1 vector, si-FBXW7 (5′-GCTCCCTAAAGAGTTGGCACTCTAT-3′) or si-Scramble (5′-GCTATCGAATGAGGTCACCTCCTAT-3′) were synthesized by Guangzhou RiboBio Co, Ltd. (Guangzhou, China). Then, SiHa and Hela cells (4 × 10⁵ cells per well) were seeded in 6-well plates and cultured overnight until reached about 80% confluence, 100 nM miR-103a-3p mimics, mimics NC, miR-103a-3p inhibitor, inhibitor NC, 2 μg pcDNA3.1-FBXW7 plasmid, and 100 ng si-FBXW7 were transfection using the Lipofectamine 3000 reagent (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol. After incubation for 6 h, regular culture medium was added into each well to terminate reaction at 37 °C. 48 post-transfection, cells were harvested for subsequent experiments.

miR-103a-3p mimics, miR-103a-3p inhibitor, PTEN siRNA, and their control fragments were obtained from GenePharma (Shanghai, China). The PTEN CDS were edited into the pcDNA3.1 vector to construct the overexpression plasmid. Transfections were performed with Lipofectamine™ 3000 Transfection Reagent (Invitrogen, Carlsbad, CA, USA).