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Donkey anti mouse af488

Manufactured by Jackson ImmunoResearch
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Donkey-anti-Mouse (AF488) is a secondary antibody conjugated with Alexa Fluor 488 dye. It is designed to detect and bind to primary antibodies raised in mouse.

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Lab products found in correlation

Donkey anti rat af647, by Jackson ImmunoResearch (2 mentions) Af647 donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Donkey anti rabbit af594, by Jackson ImmunoResearch (1 mentions) Mouse anti ctbp2, by BD (1 mentions) Mouse anti gria2, by Merck Group (1 mentions) Donkey anti goat af647, by Jackson ImmunoResearch (1 mentions) Af488 goat anti mouse, by Jackson ImmunoResearch (1 mentions) Axio scan z1, by Zeiss (1 mentions) New fluorophores, by Advanced Cell Diagnostics (1 mentions) Donkey anti rat 647, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

AF488 AffiniPure donkey anti-mouse IgG (2 citations)

4 protocols using donkey anti mouse af488

1

Immunofluorescence Antibody Protocol

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The following primary antibodies were used: mouse IgG1 anti-SIRT3 antibody (1:200; Novus Biologicals; RRID:AB_2818991); goat anti-Oncomodulin antibody (OCM; 1:1000; Santa Cruz; RRID:AB_2267583), rabbit anti-Myosin7a (MYO7; 1:200; Proteus; RRID:AB_10013626) mouse anti-CTBP2 (aka C-Terminal Binding Protein 2; 1:200; BD Transduction Laboratories; RRID:AB_399431), and mouse anti-GRIA2 (aka GluR2/GluA2; 1:2000; Millipore; RRID:AB_2113875). The following secondary antibodies were purchased from Jackson Immuno Research: Donkey Anti-Mouse AF488 (1:500; RRID:AB_2340849), Donkey Anti-Rabbit AF594 (1:500; RRID:AB_2340622), Donkey Anti-Rabbit AF647 (1:200; RRID:AB_2340625), Donkey Anti-Goat AF647 (1:200; RRID:AB_2340438), Goat Anti-Mouse AF594 (IgG1, 1:500; RRID:AB_2338885), AF488 Goat Anti-Mouse (IgG2a, 1:500; RRID:AB_2338855). For the images in Fig 1, an AF568 Goat Anti-Mouse (IgG1, 1:200, Thermo Fisher, RRID: AB_2535766) was used.
Patel S., Shah L., Dang N., Tan X., Almudevar A, & White P.M. (2020). SIRT3 promotes auditory function in young adult FVB/nJ mice but is dispensable for hearing recovery after noise exposure. PLoS ONE, 15(7), e0235491.
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2

Immunostaining of Drosophila Brains

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Brains were dissected in PBS and fixed in 4%PFA + 0.5% Triton X-100 (Triton) at RT for 20min, nutating. Brains were rinsed 2x with PBST (0.5% Triton X-100) at RT, then washed 1x with 0.5% PBSTriton at RT for 30min, nutating. For primary antibody staining, brains were incubated in Starting Block + 0.5% Triton at RT for 30min. Antibodies were then added and the brains were incubated at 4C overnight, nutating. Brains were then washed as described above followed by incubating in Starting Block + 0.5% Triton at RT for 30min. Secondary antibodies were added and brains were incubated at RT for 2hr, protected from light. Brains were finally rinsed 2X in PBST (0.5% Triton X-100) at RT for 1min each, then washed 2X in PBS for 30min. Brains were subsequently mounted as described in the HCR section above.
Antibodies were diluted as the following: Mouse-anti-Fas3 1:50 (DHSB), Rat-anti-dpn (1:1000) (C-YL lab), Donkey-anti-Mouse (AF488) 1:500 (Jackson ImmunoResearch Laboratories, Inc.), Donkey-anti-Rat (AF647) 1:500 (Jackson ImmunoResearch Laboratories, Inc.).
Michki N.S., Li Y., Sanjasaz K., Zhao Y., Shen F.Y., Walker L.A., Cao W., Lee C.Y, & Cai D. (2021). The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis. Cell reports, 35(4), 109039.
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3

Immunostaining of Drosophila Brains

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Brains were dissected in PBS and fixed in 4%PFA + 0.5% Triton X-100 (Triton) at RT for 20min, nutating. Brains were rinsed 2x with PBST (0.5% Triton X-100) at RT, then washed 1x with 0.5% PBSTriton at RT for 30min, nutating. For primary antibody staining, brains were incubated in Starting Block + 0.5% Triton at RT for 30min. Antibodies were then added and the brains were incubated at 4C overnight, nutating. Brains were then washed as described above followed by incubating in Starting Block + 0.5% Triton at RT for 30min. Secondary antibodies were added and brains were incubated at RT for 2hr, protected from light. Brains were finally rinsed 2X in PBST (0.5% Triton X-100) at RT for 1min each, then washed 2X in PBS for 30min. Brains were subsequently mounted as described in the HCR section above.
Antibodies were diluted as the following: Mouse-anti-Fas3 1:50 (DHSB), Rat-anti-dpn (1:1000) (C-YL lab), Donkey-anti-Mouse (AF488) 1:500 (Jackson ImmunoResearch Laboratories, Inc.), Donkey-anti-Rat (AF647) 1:500 (Jackson ImmunoResearch Laboratories, Inc.).
Michki N.S., Li Y., Sanjasaz K., Zhao Y., Shen F.Y., Walker L.A., Cao W., Lee C.Y, & Cai D. (2021). The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis. Cell reports, 35(4), 109039.
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4

Multispectral Imaging of Tissue Samples

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After each round of HiPlex RNAscope hybridization, fluorescent images were acquired on Axio Scan-Z1 (Zeiss) fluorescent slide scanner. Whole slide images were collected with a 40x/0.95 objective, with a 0.5 μm step size. Images for each individual in the study were collected from at least 2–4 salivary glands. After each round of imaging for HiPlex RNAscope, coverslips were removed by submerging the slides in SSC 5X buffer until coverslips fall off the slide. Subsequently, fluorophores were cleaved, autofluorescence quenched, and then new fluorophores were added (Advanced Cell Diagnostics, Newark, CA, USA). Immediately after imaging was repeated on the same tissue slices using the additional ISH probes. We performed this for three rounds of RNAscope, after final ISH imaging step, fluorophores were cleaved, autofluorescence quenched and the following secondary antibodies were added: donkey-antiMouseAF488, donkey-antiRabbitAF446 and donkey-antiRat647, Jackson Immunoresearch, all in dilution 1:300 in Co-detection Antibody Diluent from ACD. Finally, antibodies were washed in PBS-T and mounted in prolong-Diamond antifade with DAPI. A final round of imaging was performed to detect the signal of the secondary antibodies.
Warner B., Pranzatelli T., Perez P., Ku A., Matuck B.F., Huynh K., Sakai S., Abed M., Jang S.I., Yamada E., Dominick K., Ahmed Z., Oliver A., Wasikowski R., Easter Q., Magone M.T., Baer A., Pelayo E., Khavandgar Z., Gupta S., Kleiner D., Lessard C., Farris A., Martin D., Morell R., Zheng C., Rachmaninoff N., Maldonado-Ortiz J., Qu X., Aure M., Dezfulian M., Lake R., Teichmann S., Barber D., Tsoi L., Sowalsky A., Tyc K., Gudjonsson J., Byrd K., Johnson P., Liu J, & Chiorini J. (2023). GZMK+CD8+ T cells Target a Specific Acinar Cell Type in Sjögren’s Disease. Research Square.
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