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2 protocols using authentic standard 7 keto cholesterol

1

Quantification of Oxysterol Biomarkers

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Authentic standards (24(S)-hydroxycholesterol, 27-hydroxycholesterol, 25-hydroxycholesterol, 7ß-hydroxycholesterol) and deuterated (24(R/S)-hydroxycholesterol-d7, 25-hydroxycholesterol-d6, 27-hydroxycholesterol-d6, 7ß-hydroxycholesterol-d7, 7-ketocholesterol-d7) were purchased from Avanti polar lipids, Alabama. Authentic standard 7 keto cholesterol was purchased from Cayman chemicals, MI, USA. Butylacetate, hexane, isopropanol, methanol and formic acid (HPLC/MS grade) were purchased from Fisher Scientific, UK. Butylated hydroxytoluene (BHT) and Discovery DSC18 cartridges were from Sigma-Aldrich, UK. Oasis HLB Prime and Oasis HLB cartridges were purchased from Waters.
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2

Quantitative Analysis of Oxysterols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Authentic standards (24(S)-hydroxycholesterol, 26-hydroxycholesterol, 25-hydroxycholesterol, 7β-hydroxycholesterol) and deuterated (24(R/S)-hydroxycholesterol-d7, 25-hydroxycholesterol-d6, 26-hydroxycholesterol-d6, 7β-hydroxycholesterol-d7, 7-ketocholesterol-d5) were purchased from Avanti polar lipids, Alabama. Authentic standard 7 keto cholesterol was purchased from Cayman chemicals, MI, USA. Butyl acetate, hexane, isopropanol, methanol, and formic acid (HPLC/MS grade) were purchased from Fisher Scientific, UK. Butylated hydroxytoluene (BHT) was from Sigma-Aldrich, UK. Oasis HLB Prime cartridges were purchased from Waters.
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