To examine the percentages of ST2+ cells, cells were suspended in PBS and incubated with PE-labeled anti-ST2 antibody (Biolegend) for 30 min. Cell apoptosis was measured using the Annexin V apoptosis detection kit (eBioscience) and in situ apoptosis detection kit (Roche) according to the manufacturer’s directions. For analysis of phosphorylation AKT-S473, cells were fixed and permeabilized with the BD Cytofix/Cytoperm kit (BD Biosciences) and then stained with mouse anti-phospho-AKT-S473 PE or isotype control (eBioscience). For analysis of RET, PUMA, or p53 protein levels, cells were fixed in 4% paraformaldehyde and permeabilized in 0.25% saponin. Cells were stained with a primary anti-RET, anti-PUMA or anti-p53 antibody (MultiSciences Biotech Co., Ltd, Hangzhou, China) followed by a secondary donkey anti-rabbit PE antibody (Biolegend). Flow cytometric analysis was performed with FACSCalibur cytometer (BD Biosciences) and CellQuest v3.3 software.
Mouse anti phospho akt s473 pe or isotype control
Manufactured by Thermo Fisher Scientific
The Mouse anti-phospho-AKT-S473 PE or isotype control is a laboratory reagent designed for flow cytometry applications. It contains a fluorescently-labeled antibody that binds to the phosphorylated form of the AKT protein at serine 473. This product can be used to detect and quantify the level of activated AKT in cells.
Automatically generated - may contain errors
Lab products found in correlation
Annexin 5 apoptosis detection kit, by Thermo Fisher Scientific (2 mentions)
Pe labeled anti st2 antibody, by BioLegend (2 mentions)
Facscalibur cytometer, by BD (2 mentions)
Cytofix cytoperm kit, by BD (2 mentions)
In situ apoptosis detection kit, by Roche (2 mentions)
Donkey anti rabbit pe antibody, by BioLegend (1 mentions)
Secondary donkey anti rabbit pe antibody, by BioLegend (1 mentions)
2 protocols using mouse anti phospho akt s473 pe or isotype control
1
Characterization of ST2+ Cells and Apoptosis Signaling
2
Multiparametric Flow Cytometry for Apoptosis and Signaling
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To examine the percentages of ST2 + cells, cells were suspended in PBS and incubated with PE-labeled anti-ST2 antibody (Biolegend) for 30 min. Cell apoptosis was measured using the Annexin V apoptosis detection kit (eBioscience) and in situ apoptosis detection kit (Roche) according to the manufacturer's directions. For analysis of phosphorylation AKT-S473, cells were fixed and permeabilized with the BD Cytofix/Cytoperm kit (BD Biosciences), and then stained with mouse anti-phospho-AKT-S473 PE or isotype control (eBioscience). For analysis of RET, PUMA or p53 protein levels, cells were fixed in 4% paraformaldehyde and permeabilized in 0.25% saponin. Cells were stained with a primary anti-RET, anti-PUMA or anti-p53 antibody (MultiSciences Biotech Co., Ltd, Hangzhou, China) followed by a secondary donkey anti-rabbit PE antibody (Biolegend). Flow cytometric analysis was performed with FACSCalibur cytometer (BD Biosciences) and CellQuest v3.3 software.
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