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Anti irf3 rabbit polyclonal

Manufactured by Santa Cruz Biotechnology
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Anti-IRF3 rabbit polyclonal is a primary antibody product produced in rabbits and specific to the interferon regulatory factor 3 (IRF3) protein. It is designed for research applications involving the detection and study of IRF3.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Keratinocyte serum free media, by Thermo Fisher Scientific (2 mentions) Anti sting rabbit polyclonal, by Cell Signaling Technology (2 mentions) Anti mavs rabbit polyclonal, by Enzo Life Sciences (2 mentions) Anti traf3 rabbit polyclonal, by Cell Signaling Technology (2 mentions) Anti p65 rabbit polyclonal, by Santa Cruz Biotechnology (2 mentions) Miridian human microrna mimic library mirbase version 10.1, by Horizon Discovery (2 mentions) Non targeting control caenorhabditis elegans mirnas and sirnas, by Horizon Discovery (2 mentions) Mouse monoclonal anti β actin antibody, by Merck Group (2 mentions) Recombinant human ifnβ, by R&D Systems (2 mentions)

Spelling variants of this product

Anti-IRF3 (27 citations) Polyclonal rabbit (4 citations) Rabbit-anti-IRF3 (2 citations) Rabbit anti-TM (2 citations) Anti-IRF3 rabbit polyclonal antibody (2 citations)

2 protocols using anti irf3 rabbit polyclonal

1

Screening of miRNA Modulators of STING Pathway

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Immortalized human bronchial epithelial cells (HBEC) were obtained from J. Minna (UT Southwestern Medical Center) and were cultured in keratinocyte serum-free media (Invitrogen) supplemented with EGF and bovine pituitary extract. Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Invitrogen) with 10% FBS (Atlas Biologicals). The following antibodies were used for immunoblot analysis according to the manufacturer’s instructions: anti-STING rabbit polyclonal (Cell Signaling), anti-MAVS rabbit polyclonal (Enzo Life Sciences), anti-TRAF3 rabbit polyclonal (Cell Signaling Technology), anti-P65 rabbit polyclonal (Santa Cruz), anti-IRF3 rabbit polyclonal (Santa Cruz), and anti-β-actin mouse monoclonal antibodies (Sigma). Antibodies against VSV proteins were used as previously reported25 (link). The miRIDIAN human microRNA mimic library (miRBase version 10.1) was obtained from Dharmacon, Thermo. A total of 810 mimics were screened, which represented 723 unique mature miRNAs. Non-targeting control Caenorhabditis elegans miRNAs and siRNAs were purchased from Dharmacon. Recombinant human IFNβ was obtained from R&D systems.
Yarbrough M.L., Zhang K., Sakthivel R., Forst C.V., Posner B.A., Barber G.N., White M.A, & Fontoura B.M. (2014). Primate-Specific miR-576-3p Sets Host Defense Signaling Threshold. Nature communications, 5, 4963.
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2

Screening of miRNA Modulators of STING Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immortalized human bronchial epithelial cells (HBEC) were obtained from J. Minna (UT Southwestern Medical Center) and were cultured in keratinocyte serum-free media (Invitrogen) supplemented with EGF and bovine pituitary extract. Human embryonic kidney (HEK) 293T cells were cultured in DMEM (Invitrogen) with 10% FBS (Atlas Biologicals). The following antibodies were used for immunoblot analysis according to the manufacturer’s instructions: anti-STING rabbit polyclonal (Cell Signaling), anti-MAVS rabbit polyclonal (Enzo Life Sciences), anti-TRAF3 rabbit polyclonal (Cell Signaling Technology), anti-P65 rabbit polyclonal (Santa Cruz), anti-IRF3 rabbit polyclonal (Santa Cruz), and anti-β-actin mouse monoclonal antibodies (Sigma). Antibodies against VSV proteins were used as previously reported25 (link). The miRIDIAN human microRNA mimic library (miRBase version 10.1) was obtained from Dharmacon, Thermo. A total of 810 mimics were screened, which represented 723 unique mature miRNAs. Non-targeting control Caenorhabditis elegans miRNAs and siRNAs were purchased from Dharmacon. Recombinant human IFNβ was obtained from R&D systems.
Yarbrough M.L., Zhang K., Sakthivel R., Forst C.V., Posner B.A., Barber G.N., White M.A, & Fontoura B.M. (2014). Primate-Specific miR-576-3p Sets Host Defense Signaling Threshold. Nature communications, 5, 4963.
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