Cells were dissociated into single cells with trypsin, washed once in PBS, resuspended in 300 μL PBS, and fixed by dropwise addition of 700 μL pre-chilled (‒20°C) 100% ethanol. After fixation at 4°C or storage at 20°C, cells were washed once with 6 mL 1% BSA/PBS and pelleted by centrifuge. Cell pellets were loosed and resuspended by flick in 100 μL 1% BSA/PBS with 1 μL Alexa Fluor 488 conjugated phospho-Histone H3 (Ser10) antibody (DC28, Cell Signaling). After incubation at room temperature for 1 hour with occasional flick, cells were washed once with 6 mL 1% BSA/PBS and resuspended in PBS containing 10 μg/ml propidium iodide (Santa Cruz) and 100 μg/ml RNase A (QIAGEN). Flow cytometry was performed on a BD FACSCalibur and analyzed with FlowJo software. Single cell population and G1/S/G2 population were manually gated.
Alexa fluor 488 conjugated phospho histone h3 ser10 antibody dc28
Manufactured by Cell Signaling Technology
The Alexa Fluor 488 conjugated phospho-Histone H3 (Ser10) antibody (DC28) is a fluorescently labeled antibody that specifically recognizes histone H3 phosphorylated at serine 10.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using alexa fluor 488 conjugated phospho histone h3 ser10 antibody dc28
1
Cell Cycle Analysis by Flow Cytometry
2
Cell Cycle Analysis by Flow Cytometry
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Cells were dissociated into single cells with trypsin, washed once in PBS, resuspended in 300 μL PBS, and fixed by dropwise addition of 700 μL pre-chilled (‒20°C) 100% ethanol. After fixation at 4°C or storage at 20°C, cells were washed once with 6 mL 1% BSA/PBS and pelleted by centrifuge. Cell pellets were loosed and resuspended by flick in 100 μL 1% BSA/PBS with 1 μL Alexa Fluor 488 conjugated phospho-Histone H3 (Ser10) antibody (DC28, Cell Signaling). After incubation at room temperature for 1 hour with occasional flick, cells were washed once with 6 mL 1% BSA/PBS and resuspended in PBS containing 10 μg/ml propidium iodide (Santa Cruz) and 100 μg/ml RNase A (QIAGEN). Flow cytometry was performed on a BD FACSCalibur and analyzed with FlowJo software. Single cell population and G1/S/G2 population were manually gated.
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