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Polyclonal rabbit hsf1

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Polyclonal rabbit HSF1 is a laboratory reagent used to detect the transcription factor Heat Shock Factor 1 (HSF1) in various experimental applications. HSF1 is a key regulator of the cellular heat shock response and plays a crucial role in the expression of genes encoding heat shock proteins.

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Lab products found in correlation

Monoclonal rabbit ps326 hsf1, by Abcam (2 mentions) Monoclonal mouse hsp90ab1, by GeneTex (2 mentions) Monoclonal rabbit hspa8, by Cell Signaling Technology (2 mentions) Monoclonal rabbit notch1, by Cell Signaling Technology (2 mentions) Monoclonal mouse actin, by Merck Group (2 mentions) Polyclonal goat lamin a, by Santa Cruz Biotechnology (2 mentions)

2 protocols using polyclonal rabbit hsf1

1

Evaluating HSF1 Regulatory Targets in T-ALL

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against the following proteins were used: polyclonal rabbit HSF1 (Cell Signaling, 4356), monoclonal rabbit pS326 HSF1 (Abcam, 76076), monoclonal mouse HSP90AB1 (GeneTex, 84342), monoclonal rabbit HSPA8 (Cell signaling, 8444), monoclonal rabbit NOTCH1 (Cell Signaling, 4147), monoclonal mouse Actin (Millipore, MAB1501) and polyclonal goat Lamin A (Santa Cruz biotechnology, sc-6214). The shRNAs used against HSF1 and HSF1 positively regulated targets are described in Supplementary Table 3. To generate virus, we transfected HEK293T cells with a plasmid (pLKO.1) expressing the shRNA of interest and lentiviral packaging plasmids. Viral supernatant was collected over a period of 72 h and used for the transduction of T-ALL cells. For survival assays, the cells were infected twice and selected with puromycin starting 2 days after viral infection. For RNA-seq experiments and to avoid the effects of apoptosis due to HSF1 knockdown, infection conditions were optimized to reach ~90% transduction efficiency and RNA was collected 48 h after infection.
Kourtis N., Lazaris C., Hockemeyer K., Balandrán J.C., Jimenez A.R., Mullenders J., Gong Y., Trimarchi T., Bhatt K., Hu H., Shrestha L., Ambesi-Impiombato A., Kelliher M., Paietta E., Chiosis G., Guzman M.L., Ferrando A.A., Tsirigos A, & Aifantis I. (2018). Oncogenic hijacking of the stress response machinery in T cell acute lymphoblastic leukemia. Nature medicine, 24(8), 1157-1166.
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2

Evaluating HSF1 Regulatory Targets in T-ALL

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against the following proteins were used: polyclonal rabbit HSF1 (Cell Signaling, 4356), monoclonal rabbit pS326 HSF1 (Abcam, 76076), monoclonal mouse HSP90AB1 (GeneTex, 84342), monoclonal rabbit HSPA8 (Cell signaling, 8444), monoclonal rabbit NOTCH1 (Cell Signaling, 4147), monoclonal mouse Actin (Millipore, MAB1501) and polyclonal goat Lamin A (Santa Cruz biotechnology, sc-6214). The shRNAs used against HSF1 and HSF1 positively regulated targets are described in Supplementary Table 3. To generate virus, we transfected HEK293T cells with a plasmid (pLKO.1) expressing the shRNA of interest and lentiviral packaging plasmids. Viral supernatant was collected over a period of 72 h and used for the transduction of T-ALL cells. For survival assays, the cells were infected twice and selected with puromycin starting 2 days after viral infection. For RNA-seq experiments and to avoid the effects of apoptosis due to HSF1 knockdown, infection conditions were optimized to reach ~90% transduction efficiency and RNA was collected 48 h after infection.
Kourtis N., Lazaris C., Hockemeyer K., Balandrán J.C., Jimenez A.R., Mullenders J., Gong Y., Trimarchi T., Bhatt K., Hu H., Shrestha L., Ambesi-Impiombato A., Kelliher M., Paietta E., Chiosis G., Guzman M.L., Ferrando A.A., Tsirigos A, & Aifantis I. (2018). Oncogenic hijacking of the stress response machinery in T cell acute lymphoblastic leukemia. Nature medicine, 24(8), 1157-1166.
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