Fresh whole rat blood was mixed in a 10:1 volume ratio of an acid citrate dextrose (ACD) anticoagulant buffer (containing 2.13% free citrate ion, BD Vacutainer Specialty Venous Blood Collection Tubes) for the preparation of platelet-rich plasma (PRP). PRP was obtained via centrifugation at 600 x g for 10 min at 10 °C. The platelets were counted using a Countess II Automated Cell Counter (ThermoFisher Scientific) and diluted to 2.5 x 106 platelets/mL in 1x PBS. 6 mm punches of hydrogels were placed in ultra-low adhesion 96-well plates and incubated for 24 hr at 37 °C. Gels were UV sterilized for 5 min prior to incubation with platelets. 100 μL of PRP was pipetted on top of the hydrogels. The plates were placed on a rotary shaker for 1 hr at room temperature. Platelets were rinsed once with 1x PBS and fixed with 4% paraformaldehyde (PFA). Cells were imaged with an EVOS XL Core Imaging System microscope (Life Technologies).
Evos xl core imaging system microscope
Manufactured by Thermo Fisher Scientific
The EVOS XL Core Imaging System is a compact, benchtop microscope designed for brightfield and fluorescence imaging. It features LED illumination, a high-resolution camera, and user-friendly software for image capture and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using evos xl core imaging system microscope
1
Platelet Adhesion to Hydrogel Scaffolds
2
Platelet Adhesion on Hydrogel Surfaces
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Platelet assay was performed as described previously 27 . Briefly, 6 mm punches of hydrogels were incubated for 24 hr at 37 °C with 50% fetal bovine serum (FBS). Then, whole rat blood was mixed with acid citrate dextrose (ACD) anticoagulant buffer for the preparation of platelet-rich plasma (PRP). PRP was obtained via centrifugation at 600 g for 10 min at 10°C.
Platelets were diluted to 2.5 x 10 6 platelets/mL in 1x phosphate buffered saline (PBS). 100 µL of the platelet rich plasma was incubated for 1 hour on hydrogel surfaces. Platelets were rinsed with 1x PBS and fixed with 4% paraformaldehyde (PFA). Hydrogel surfaces were imaged with an EVOS XL Core Imaging System microscope (Life Technologies).
Platelets were diluted to 2.5 x 10 6 platelets/mL in 1x phosphate buffered saline (PBS). 100 µL of the platelet rich plasma was incubated for 1 hour on hydrogel surfaces. Platelets were rinsed with 1x PBS and fixed with 4% paraformaldehyde (PFA). Hydrogel surfaces were imaged with an EVOS XL Core Imaging System microscope (Life Technologies).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!