Human il 1β and caspase 1 p20 elisa kits

Manufactured by R&D Systems

Sourced in United States

The Human IL-1β and caspase-1 (p20) ELISA kits are quantitative assay kits for the measurement of human interleukin-1 beta (IL-1β) and caspase-1 (p20) levels in cell culture supernatants, plasma, and serum samples. These kits utilize the quantitative sandwich enzyme immunoassay technique.

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Lab products found in correlation

Anti β actin antibody, by Santa Cruz Biotechnology (2 mentions) Anti nlrp 3 antibody, by Merck Group (2 mentions) Stat 3 tyr705, by Cell Signaling Technology (1 mentions) Recombinant human gm csf, by Thermo Fisher Scientific (1 mentions) Tofacitinib, by Merck Group (1 mentions) Iberiotoxin, by Merck Group (1 mentions) Hydroxychloroquine, by Merck Group (1 mentions) Adenosine 5 triphosphate, by Merck Group (1 mentions) Recombinant human saa, by Thermo Fisher Scientific (1 mentions) Anti phospho nf κb p65 ser536 antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Human IL-1β ELISA kit Human IL-1β IL-1β Human kits Caspase-1 ELISAs

2 protocols using human il 1β and caspase 1 p20 elisa kits

JAK-STAT Signaling in Inflammation

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Recombinant human GM-CSF was purchased from Peprotech (Rocky Hills, NJ). Anti-β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, USA). Anti-NLRP-3 antibody was purchased from MERCK MILLIPORE (Billerica, MA USA). Human IL-1β and caspase-1 (p20) ELISA kits were purchased from R&D systems (Minneapolis, USA). Phospho-specific antibodies against JAK-2 (Tyr1007/1008), STAT-5 (Tyr701) and STAT-3 (Tyr705) were purchased from Cell Signaling Technology (Beverly, MA). Tofacitinib, baricinib and upadacitinib were purchased from Sigma-Aldrich (Tokyo, Japan).

Fujita Y., Matsuoka N., Temmoku J., Furuya-Yashiro M., Asano T., Sato S., Matsumoto H., Watanabe H., Kozuru H., Yatsuhashi H., Kawakami A, & Migita K. (2020). JAK inhibitors impair GM-CSF-mediated signaling in innate immune cells. BMC Immunology, 21, 35.

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Recombinant SAA Modulation of NLRP3

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Recombinant human SAA was purchased from Peprotech (Rocky Hills, NJ). The endotoxin levels were less than 0.1 ng/μg protein. Anti-β-actin antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, USA). Anti-NLRP-3 antibody was purchased from MERCK MILLIPORE (Billerica, MA, USA). Human IL-1β and caspase-1 (p20) ELISA kits were purchased from R&D systems (Minneapolis, USA). Anti-phospho-NF-κB p65 (Ser536) antibody was purchased from Cell Signaling Technology (CST, Danvers, USA). Hydroxychloroquine, iberiotoxin (IBTX), lipopolysaccharide (LPS), and adenosine 5 triphosphate (ATP) were purchased from Sigma-Aldrich (Tokyo, Japan).

Fujita Y., Matsuoka N., Temmoku J., Furuya M.Y., Asano T., Sato S., Kobayashi H., Watanabe H., Suzuki E., Urano T., Kozuru H., Yatsuhashi H., Koga T., Kawakami A, & Migita K. (2019). Hydroxychloroquine inhibits IL-1β production from amyloid-stimulated human neutrophils. Arthritis Research & Therapy, 21, 250.

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