The Vα14i TCRα gene tagged with ΔNGFR was transduced into TCR-/- 5KC cells. The clone Jurkat 76.3E1 was established by serially infecting Jurkat 76 cells with the CD4, CD8αβ, and Vα14i TCRα-ΔNGFR genes as previously reported (35–37). Surface CD1d expression was knocked down with the transduction of a retroviral vector encoding a β2-microglobulin-targeting shRNA (OriGene Technologies, Rockville, MD). 5KC-expressing Vα14i TCRα and 3E1 cells were then reconstituted with the TCRβ genes. All retroviral infection of the T cell lines used 293GPG. All Jurkat 76.3E1 transfectants had >90% CD3 positivity. HLA-A2/TAX TCR (A6) was used as the control TCR [35 (link)].
Retroviral vector
Manufactured by OriGene
Sourced in United States
Retroviral vector is a laboratory tool used for gene delivery. It is a modified virus that can be used to introduce genetic material into target cells. The core function of the retroviral vector is to facilitate the transfer and integration of desired genes into the host cell's genome.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using retroviral vector
1
Generation of CD1d-restricted Vα14i TCR Cells
2
Downregulation of CD44 in OG-MEFs via Retroviral Transduction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CD44 RNA was downregulated in the OG-MEFs by retroviral transduction to introduce a predesigned CD44 mouse shRNA into the retroviral vector (Origene, USA) targeting CD44. The CD44 mouse shRNA of the retroviral vector was purchased in annealed and purified form ready to be transfected into the GP2-293 packaging cells for retroviral synthesis. For the transduction, 1 × 106 cells were plated in 100 mm dish, and the adhered cells were transduced with retrovirus synthesized using polybrene. The OG-MEFs were transduced with CD44 mouse shRNA for 24 h and then replaced with new growth medium.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!