Brewers yeast

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

Brewers' yeast is a type of yeast used in the production of beer and other alcoholic beverages. It is a single-celled fungus that ferments the sugars in the wort or must, producing alcohol and carbon dioxide as by-products. Brewers' yeast is an important component in the brewing process, as it is responsible for the conversion of fermentable sugars into alcohol.

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Lab products found in correlation

Neomycin sulfate, by Thermo Fisher Scientific (1 mentions) L ascorbic acid, by Thermo Fisher Scientific (1 mentions) 1 octacosanol, by Merck Group (1 mentions) Methyl p hydroxybenzoate, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Acetone, by Merck Group (1 mentions) Formaldehyde, by Merck Group (1 mentions) Morphine, by Merck Group (1 mentions) Win55 212 2, by Bio-Techne (1 mentions) Capsaicin, by Merck Group (1 mentions) Indomethacin, by Merck Group (1 mentions)

4 protocols using brewers yeast

Drosophila Rearing on Standard and Lyophilized Diets

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Flies were reared on standard sugar/yeast/agar food (standard food) or Nutri‐Fly food, based on the experimental setup. Standard food consists of 85 g corn flour, 60 g sugar, 30 g brewer's yeast (ACROS Organics™, cat. # 368080050), 10 g Insectagar ZN5 (B.&V. S.R.L. The Agar Company), 50 g Molasses, 15 ml/l 1 M Propionic Acid (Genesee Scientific, cat. #789177), and 15 ml/l 10% w/v Tegosept in EtOH 96% (Genesee Scientific, cat. #789063) per liter.
Lyophilized food consists of 200 g Nutri‐Fly food (Genesee Scientific, cat. #789211) and 16.25 g brewer's yeast (ACROS Organics™, cat. # 368080050) per liter.

Aiello G., Sabino C., Pernici D., Audano M., Antonica F., Gianesello M., Ballabio C., Quattrone A., Mitro N., Romanel A., Soldano A, & Tiberi L. (2022). Transient rapamycin treatment during developmental stage extends lifespan in Mus musculus and Drosophila melanogaster. EMBO Reports, 23(9), e55299.

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Fly Genetics and Crosses for Research

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Fly cultures and crosses were raised at 25°C on a standard medium containing 9 g/L agar (ZN5 B and V), 75 g/L corn flour, 60 g/L white sugar, 30 g/L brewers’ yeast (Fisher Scientific), 50 g/L fresh yeast and 50 mL/L molasses (Naturitas), along with nipagin and propionic acid (Fisher). The lines used were obtained by: UAS-HA-MYC (Bellosta et al., 2005 (link)
); NOC1-GFP (B51967) UAS-NOC1-HA (Flyorf-CH) NOC1-RNAi (B25992). UAS-Ns1-GFP is a gift from Patrick J. Di Mario University of Louisiana, LA). hsp70-Gal4 gift from Florenci Serras (University of Barcelona, Spain).

Manara V., Radoani M., Belli R., Peroni D., Destefanis F., Angheben L., Tome G., Tebaldi T, & Bellosta P. (2023). NOC1 is a direct MYC target, and its protein interactome dissects its activity in controlling nucleolar function. Frontiers in Cell and Developmental Biology, 11, 1293420.

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Analytical-Grade Reagents Protocol

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All reagents used in this study were of analytical grade and commercially available. Agar, brewer's yeast, L-ascorbic acid, and neomycin sulfate were purchased from Fisher Scientific (Thermo-Fisher Scientific, Waltham, Massachusetts, USA); acetone, ethanol, formaldehyde, methyl p-hydroxybenzoate, 1-octacosanol were from Sigma-Aldrich (St. Louis, Missouri, USA).

Zavala-Sánchez M.Á., Rodríguez‐Chávez J.L., Figueroa‐Brito R., Quintana-López C.M., Bah M.M., Campos‐Guillén J., Bustos‐Martínez J., Zamora-Avella D., & Ramos-López M.Á. (2020). Bioactivity of 1-octacosanol from Senna crotalarioides (Fabaceae: Caesalpinioideae) to Control Spodoptera frugiperda (Lepidoptera: Noctuidae). Florida Entomologist, 102(4), 731-731.

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Pharmacological Preparations for Pain Research

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Acetaminophen (APAP, 4-Acetamidophenol; Sigma-Aldrich, UK) and morphine (Sigma-Aldrich, UK) were prepared in 0.9% saline (with warming for APAP). WIN55,212-2 (Tocris, UK) was made up in 5% DMSO/5% Tween 80/0.9% saline, RTX (Sigma-Aldrich, UK) in 10% ethanol/10% Tween 80/saline, indomethacin (Sigma-Aldrich, UK) in 5% sodium hydrogen carbonate (pH to 7.4), cinnamaldehyde (Fluka) in 0.5% Tween 80/saline and brewer’s yeast; (Acros Organics) in 0.9% saline. BCTP (7-tert-butyl-6-(4-chloro-phenyl)-2-thioxo-2,3-dihydro-1H-pyrido[2,3-d]pyrimidin-4-one) was suspended in 0.5% methyl cellulose. CHEM5861528 (Chembridge Corporation, USA), capsaicin (Sigma-Aldrich, UK) was administered in 10% DMSO/saline. Mice were exposed to isoflurane (Abbott Industries Ltd. UK) at 2.5% in 95%O₂/5% CO₂ for 5 minutes.

Gentry C., Andersson D.A, & Bevan S. (2015). TRPA1 mediates the hypothermic action of acetaminophen. Scientific Reports, 5, 12771.

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