Cell lysates were electrophoresed on SDS-PAGE gels (4–15% gradient polyacrylamide gels, BioRad, Hercules, CA, USA) and blots of the gels were probed with the following antibodies: anti-IL-2Rβ Novus, Littleton, CO, USA), anti-TGFβRII, anti-pSTAT5 (Tyr694), anti-STAT5, anti-pSmad3 (Ser423/Ser425), anti-Smad3, and anti-β-actin (all Cell Signaling, Beverly, MA, USA). The secondary Ab used were anti-rabbit IgG IRDye 800CW and anti-mouse IgG IRDye 680LT (LI-COR, Lincoln, NE, USA). Membranes were imaged using the Odyssey Infrared Imager and quantified using Image Studio v2.0 software (LI-COR).
Anti psmad3 ser423 ser425
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-pSmad3 (Ser423/Ser425) is a laboratory reagent used to detect phosphorylated Smad3 protein. Smad3 is a signaling molecule that becomes phosphorylated in response to TGF-beta receptor activation. This antibody can be used to monitor Smad3 phosphorylation in various cell-based assays.
Automatically generated - may contain errors
Lab products found in correlation
Gradient polyacrylamide gel, by Bio-Rad (2 mentions)
Anti il 2rβ novus, by Novus Biologicals (2 mentions)
Anti tgfβrii, by Cell Signaling Technology (2 mentions)
Irdye 800cw anti rabbit igg, by LI COR (2 mentions)
Anti mouse igg irdye 680lt, by LI COR (2 mentions)
Image studio v2, by LI COR (2 mentions)
Odyssey infrared imager, by LI COR (2 mentions)
Anti smad3, by Cell Signaling Technology (2 mentions)
Anti β actin, by Cell Signaling Technology (2 mentions)
2 protocols using anti psmad3 ser423 ser425
1
Western Blot Analysis of Immune Signaling
2
Western Blot Analysis of Immune Signaling
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Cell lysates were electrophoresed on SDS-PAGE gels (4–15% gradient polyacrylamide gels, BioRad, Hercules, CA, USA) and blots of the gels were probed with the following antibodies: anti-IL-2Rβ Novus, Littleton, CO, USA), anti-TGFβRII, anti-pSTAT5 (Tyr694), anti-STAT5, anti-pSmad3 (Ser423/Ser425), anti-Smad3, and anti-β-actin (all Cell Signaling, Beverly, MA, USA). The secondary Ab used were anti-rabbit IgG IRDye 800CW and anti-mouse IgG IRDye 680LT (LI-COR, Lincoln, NE, USA). Membranes were imaged using the Odyssey Infrared Imager and quantified using Image Studio v2.0 software (LI-COR).
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