The stability of the tested compounds was measured according to the procedure of Fang et al. [33 (link)] with some modification. MCH and curcumin were dissolved in 100% DMSO at 5 mM for the measurement. When measurement began, 80 μL of the tested compound solution was diluted with 4 mL of 1.5 mM phosphate-buffered solution (pH 7.4). The degradation process was followed by visible absorption spectroscopy at 410 nm in a rectangular quartz cuvette with a 1 cm optical path length at 37 °C on a Shimadzu 2600 UV/visible spectrophotometer in the presence or absence of 0.1% FBS. The absorption of the solution measured at 0 min was recorded as A0; the absorption of the solution measured at other time was recorded as At. All results are representative of three independent experiments.
2600 uv visible spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The 2600 UV/visible spectrophotometer is a laboratory instrument designed to measure the absorption or transmission of light in the ultraviolet and visible regions of the electromagnetic spectrum. It provides accurate and reliable measurements of various samples, including solutions, suspensions, and solid materials.
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Lab products found in correlation
4 protocols using 2600 uv visible spectrophotometer
1
Measurement of Compound Stability
2
UV-Vis Analysis of AE-Trypsin Complex
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UV-Vis absorption spectroscopy were studied by 2600 UV-visible Spectrophotometer (Shimadzu Corporation, Japan) with 1.0 cm quartz cell and recorded in the 185–700 nm range. Spectral analysis was performed using software in the instrument. Prepared AE–trypsin complex (molar ratio 1 : 1), mixed well, and detected after 30 minutes incubation at room temperature. Ultraviolet absorption spectra of trypsin solution (1.0 × 10−5 mol L−1), AE–trypsin complex solution and AE solution (1.0 × 10−5 mol L−1) were measured respectively. Ultrapure water was used as blank control.
3
Comprehensive Characterization of nZVI and E. Coli
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A laboratory Shanghai Leici pHS-3C pH meter (Shanghai, China) was used to monitor pH value. The morphological analysis of nZVI, biochar, and BC-nZVI samples were performed by a JEOL JEM-1011 transmission electron microscopy (TEM) (Tokyo, Japan), a FEI Quanta 600 scanning electron microscopy (SEM) (Eindhoven, the Netherlands), and a Carl Zeiss SIGMA 300 SEM (Oberkochen, Germany), respectively. The morphological analysis of E. Coli cells were performed by a JEOL JEM-1011 transmission electron microscopy (TEM) (Tokyo, Japan). The crystal morphology of nZVI samples were analyzed by Rigaku DXR-8000 X-ray diffraction (Tokyo, Japan). Concentrations of Fe(ii ) were measured at 510 nm by the Shimadzu UV-visible 2600 spectrophotometer (Kyoto, Japan). Standards containing known concentrations of ferrous ion were used as reference for each set of tests. Concentrations of Se(vi ) in solution were measured by the PerkinElmer Optima 5300 DV ICP-OES (Waltham, United States). Contents of protein and SOD in cells by Coomassie brilliant blue G250 and SOD kit.
4
Characterization of Nanoparticles
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UV-vis spectra were recorded on a Shimadzu UV-visible 2600 spectrophotometer. Morphology of nanoparticles was acquired on a field emission transmission electron microscope (FE-TEM) (Talos F200i, Thermo Scientific, Czech Republic). Particle size and zeta potential were analyzed using dynamic light scattering (DLS) (NanoBrook ZetaPALS, Brookhaven Instruments). Functional groups between 400 and 4000 cm−1 were identified by Fourier transform infrared (FTIR) spectrometry (Bruker VERTEX 70). The pH of solutions was measured with a Bante pH meter.
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