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Ultra fast sybr green qpcr master mix

Manufactured by Bio-Rad
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Ultra Fast SYBR Green QPCR Master Mix is a pre-mixed solution for performing quantitative real-time PCR (qPCR) reactions. It contains SYBR Green I dye, hot-start Taq DNA polymerase, dNTPs, and necessary buffers and salts.

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Lab products found in correlation

Brilliant 3, by Bio-Rad (2 mentions) Dneasy powerlyzer powersoil kit, by Qiagen (2 mentions) Cfx thermocycler, by Bio-Rad (2 mentions)

2 protocols using ultra fast sybr green qpcr master mix

1

Quantifying Gut Microbiome Composition

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Feces were collected from individual mice. Genomic DNA was extracted from 2 biological replicates of fecal pellets using the DNeasy PowerLyzer PowerSoil kit (Qiagen). Concentration of Pc and Bt DNA was assessed using species-specific qPCR primers (Key Resources Table). qPCR was performed using the Brilliant III, Ultra Fast SYBR Green QPCR Master Mix and a Bio Rad CFX thermocycler. Genomic DNA from Bt H-2622 and Pc H-2477 were used to generate a standard curve for each primer pair. The standard curves were used to calculate the absolute quantity of Bt or Pc DNA in the sample. The efficiency value (E) for each primer pair was calculated as 10(1/−slope) of log10(DNA input) against Ct value. qPCR index was calculated using this equation: E−Ct primer pair.
Gellman R.H., Olm M.R., Terrapon N., Enam F., Higginbottom S.K., Sonnenburg J.L, & Sonnenburg E.D. (2023). Hadza Prevotella require diet-derived microbiota-accessible carbohydrates to persist in mice. Cell reports, 42(11), 113233.
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2

Quantifying Gut Microbiome Composition

Check if the same lab product or an alternative is used in the 5 most similar protocols
Feces were collected from individual mice. Genomic DNA was extracted from 2 biological replicates of fecal pellets using DNeasy PowerLyzer PowerSoil kit (Qiagen). Concentration of Pc and Bt DNA was assessed using species-specific qPCR primers (Key Resources Table). qPCR was performed using the Brilliant III, Ultra Fast SYBR Green QPCR Master Mix and a Bio Rad CFX thermocycler. Genomic DNA from Bt H-2622 and Pc H-2477 were used to generate a standard curve for each primer pair. The standard curves were used to calculate the absolute quantity of Bt or Pc DNA in the sample. The efficiency value (E) for each primer pair was calculated as 10(1/−slope) of log10(DNA input) against Ct value. Competitive index was calculated using this equation: E−Ct
Pc primer pair/ E−Ct
Bt primer pair. A competitive index of 1 denotes equal abundance.
Gellman R.H., Olm M.R., Terrapon N., Enam F., Higginbottom S.K., Sonnenburg J.L, & Sonnenburg E.D. (2023). Hadza Prevotella Require Diet-derived Microbiota Accessible Carbohydrates to Persist in Mice. bioRxiv.
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