Inhibitor bay 11 7082

Manufactured by Merck Group

Inhibitor Bay 11-7082 is a chemical compound used in laboratory research. It functions as an inhibitor, targeting specific cellular processes. The core purpose of this product is to facilitate the study of these targeted mechanisms in a controlled experimental setting.

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Lab products found in correlation

Anti ncx1 antibody, by Abcam (1 mentions) Kb r7943, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Phorbol 12 myristate 13 acetate pma, by Merck Group (1 mentions) Recombinant human tnf α, by Thermo Fisher Scientific (1 mentions) Haecs, by Thermo Fisher Scientific (1 mentions) Tnf α, by Merck Group (1 mentions)

2 protocols using inhibitor bay 11 7082

Pancreatic Acinar Cell NF-κB Regulation

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The commercially available reagents cerulein (MCE, Shanghai, China), l-arginine (MCE), NCX1 inhibitor KB-R7943 (Sigma-Aldrich, St. Louis, Mo), nuclear factor-κB (NF-κB) inhibitor Bay 11–7082 (Sigma-Aldrich), and NF-κB activator Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) were all purchased through the indicated manufacturers. Anti-NCX1 antibody was produced by Abcam (Cambridge, UK). Antibodies against P65, pP65(ser536), GAPDH, β-actin, and goat anti-rabbit/mouse IgG (H + L) secondary antibodies were purchased from Cell Signaling Technologies (Danvers, Mass). The rat pancreatic acinar cell line AR42J and human embryonic kidney cell line HEK-293T were both supplied by the Cell Line Resource Center of the Chinese Academy of Medical Sciences (Beijing, China). All cell lines were stored frozen in liquid nitrogen and after thawing, less than 20 of passages were used over the course of 3 months for the present experiments.

Chen Y., Li W., Xie R., Tang B., Xiao Y., Yang F., Chen S., Yu S., Yang S., Dong H., Guo H, & Liu J.J. (2019). Nuclear Factor-κB Increases Intracellular Calcium by Upregulation of Na+-Ca2+ Exchanger 1 in Cerulein-Induced Acute Pancreatitis. Pancreas, 49(1), 111-119.

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Shear Stress Induced Endothelial Responses

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Human aortic endothelial cells (HAECs) (Lonza) were cultured in MCDB medium 131 (Mediatech) containing 10% fetal bovine serum (FBS), 1% L-glutamine, 1% penicillin/streptomycin, and 1% endothelial cell growth supplement (ECGS) isolated from bovine cerebral tissue. Vasoprotective and pro-remodeling shear stress profiles were generated by digitizing physiological the physiological shear stress waveforms (Dai et al., 2004 (link)). HAECs were grown to confluence in a 10cm dish, and stimulated with or without 10 ng/ml recombinant human TNFα (Invitrogen), then placed into a cone-and-plate bioreactor, and exposed to vasoprotective or pro-remodeling shear stress for 20 hours. For NF-κB inhibition studies, 5 μM of the inhibitor Bay 11–7082 (Sigma) which selectively and irreversibly inhibits NF-κB activation by blocking phosphorylation of IκBα, was added to cultures for 1 hour prior to stimulation with TNFα.

Keegan P.M., Anbazhakan S., Kang B., Pace B.S, & Platt M.O. (2016). Biomechanical and biochemical regulation of cathepsin K expression in endothelial cells converge at AP-1 and NF-κB. Biological chemistry, 397(5), 459-468.

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