Our device was designed to fit a Nikon Eclipse TE2000U microscope DIC objective slot, 16 mm (width) by 50 mm (length) by 3.6 mm (height). The microscope was equipped with Nikon CFI Plan Fluor series objectives of 5-, 40- and 100 × magnification. Schematic of the device was generated with Google SketchUp.
Cfi plan fluor series
Manufactured by Nikon
Sourced in Japan
The CFI Plan Fluor Series is a line of microscope objectives designed by Nikon. The objectives feature plan-apochromatic optics for flat field and high-contrast imaging. They are available in a range of magnifications and numerical apertures to suit various microscopy applications.
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Lab products found in correlation
3 protocols using cfi plan fluor series
1
Microscope Imaging Device Design
2
Coelomic Cells Isolation and Protein Profile
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Coelomic cells of Pontodrilus were isolated by stimulating earthworms on microscope slides, observed under a fluorescence microscope (Nikon Eclipse E600, Japan) with a 60 × objective lens (Nikon CFI Plan Fluor Series, Japan), and photographed using an attached digital camera (Nikon D5500, Japan). The fluorescence excitation was performed at 380 nm.
The crude coelomic extract of both species was run on a 15% SDS-PAGE gel using a 1D Gel Electrophoresis Mini Gel, AE-6530mPAGE (ATTO), followed by silver staining (Silver Stain MS Kit, FUJIFILM Wako Pure Chemical Corporation).
The crude coelomic extract of both species was run on a 15% SDS-PAGE gel using a 1D Gel Electrophoresis Mini Gel, AE-6530mPAGE (ATTO), followed by silver staining (Silver Stain MS Kit, FUJIFILM Wako Pure Chemical Corporation).
3
Coelomic Cell Isolation and SDS-PAGE Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Coelomic cells of Pontodrilus were isolated by stimulating earthworms on microscope slides, observed under a uorescence microscope (Nikon Eclipse E600, Japan) with a 60x objective lens (Nikon CFI Plan Fluor Series, Japan), and photographed using an attached digital camera (Nikon D5500, Japan). The uorescence excitation was 380 nm.
The protein of crude coelomic extract of both species was run by 15% SDS-PAGE gel using a 1D Gel Electrophoresis Mini Gel, AE-6530mPAGE (ATTO), followed by silver staining (Silver Stain MS Kit, FUJIFILM Wako Pure Chemical Corporation).
The protein of crude coelomic extract of both species was run by 15% SDS-PAGE gel using a 1D Gel Electrophoresis Mini Gel, AE-6530mPAGE (ATTO), followed by silver staining (Silver Stain MS Kit, FUJIFILM Wako Pure Chemical Corporation).
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