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Murine igg2b αctla4 9d9

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Murine IgG2b αCTLA4 (9D9) is a monoclonal antibody that binds to the CTLA-4 protein. CTLA-4 is a receptor expressed on T cells that regulates their activation and proliferation. The 9D9 clone of this antibody is an IgG2b isotype.

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Lab products found in correlation

Fjk 16, by Thermo Fisher Scientific (2 mentions) Cytofix cytoperm plus kit, by BD (2 mentions) Cxnft, by Thermo Fisher Scientific (2 mentions) Zombie nir, by BioLegend (2 mentions)

2 protocols using murine igg2b αctla4 9d9

1

Immune Checkpoint Therapy in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immune checkpoint therapy, rat IgG2a αPD1 (RMP1-14, Leinco)
and murine IgG2b αCTLA4 (9D9, Leinco Technologies) were used. Mice were
injected intraperitoneally with 200μg of each antibody on 3, 6 and 9 days
post tumor transplant. Antibodies used for multi-color flow cytometry were CD45
(30-F11), CD11b (M1/70), Thy1.2 (30H12), CD4 (RM4-5), CD8β (YTS156.7.7),
I-E/I-A (M5/114.15.2), CD64 (X54-5/7.1), Ly6G (1A8), T-BET (4B10), CD150/SLAM
(TC15-12F12.2), KLRG1 (2F1), ICOS (15F9), CD44 (IM7), PD-1 (29F.1A12),
SIINFEKL-H-2-Kb (25-D1.16) (BioLegend), CD24 (M1/69), F4/80
(T45-2342) (BD Biosciences), FOXP3 (FJK-16s, eBiosciences) and iNOS (CXNFT,
Invitrogen). Zombie NIR (BioLegend) was used to stain for cellular viability.
The BD Cytofix/Cytoperm Plus kit (BD Biosciences) was used following
manufacturer’s protocol for intracellular staining of iNOS, T-BET and
FOXP3.
Alspach E., Lussier D.M., Miceli A.P., Kizhvatov I., DuPage M., Luoma A.M., Meng W., Lichti C.F., Esaulova E., Vomund A.N., Runci D., Ward J.P., Gubin M.M., Medrano R.F., Arthur C.D., White J.M., Sheehan K.C., Chen A., Wucherpfennig K.W., Jacks T., Unanue E.R., Artyomov M.N, & Schreiber R.D. (2019). MHC-II neoantigens shape tumor immunity and response to immunotherapy. Nature, 574(7780), 696-701.
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2

Immune Checkpoint Therapy in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immune checkpoint therapy, rat IgG2a αPD1 (RMP1-14, Leinco)
and murine IgG2b αCTLA4 (9D9, Leinco Technologies) were used. Mice were
injected intraperitoneally with 200μg of each antibody on 3, 6 and 9 days
post tumor transplant. Antibodies used for multi-color flow cytometry were CD45
(30-F11), CD11b (M1/70), Thy1.2 (30H12), CD4 (RM4-5), CD8β (YTS156.7.7),
I-E/I-A (M5/114.15.2), CD64 (X54-5/7.1), Ly6G (1A8), T-BET (4B10), CD150/SLAM
(TC15-12F12.2), KLRG1 (2F1), ICOS (15F9), CD44 (IM7), PD-1 (29F.1A12),
SIINFEKL-H-2-Kb (25-D1.16) (BioLegend), CD24 (M1/69), F4/80
(T45-2342) (BD Biosciences), FOXP3 (FJK-16s, eBiosciences) and iNOS (CXNFT,
Invitrogen). Zombie NIR (BioLegend) was used to stain for cellular viability.
The BD Cytofix/Cytoperm Plus kit (BD Biosciences) was used following
manufacturer’s protocol for intracellular staining of iNOS, T-BET and
FOXP3.
Alspach E., Lussier D.M., Miceli A.P., Kizhvatov I., DuPage M., Luoma A.M., Meng W., Lichti C.F., Esaulova E., Vomund A.N., Runci D., Ward J.P., Gubin M.M., Medrano R.F., Arthur C.D., White J.M., Sheehan K.C., Chen A., Wucherpfennig K.W., Jacks T., Unanue E.R., Artyomov M.N, & Schreiber R.D. (2019). MHC-II neoantigens shape tumor immunity and response to immunotherapy. Nature, 574(7780), 696-701.
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