Cells were cultured in medium containing 10 μM EdU (Beyotime) for 2 h and then collected and fixed with 4% paraformaldehyde. After washing with PBS, cells were treated with 0.5% Triton X-100 for 10 min at room temperature, and were then incubated with 1 × click buffer at room temperature for 30 min in the dark. Cells were capped using fluorescent mounting medium with DAPI (ZSGB Bio, China), and captured using a fluorescence microscope (Olympus). For the immunofluorescence assay, CRC cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100. After blocking with 10% goat serum, the cells were incubated with Ki67 antibodies (1:200, Abclonal, China) at 4°C overnight. These samples were then incubated with the corresponding secondary antibodies and capped using fluorescent mounting medium with DAPI (ZSGB Bio). Images were captured by a fluorescence microscope (Olympus).
Fluorescent mounting medium with dapi
Manufactured by ZSGB-BIO
Sourced in China
Fluorescent mounting medium with DAPI is a solution designed for use in fluorescence microscopy. It contains the nuclear stain DAPI (4',6-diamidino-2-phenylindole), which binds to DNA and emits blue fluorescence when excited with ultraviolet light. This product is primarily used to mount and preserve fluorescently labeled samples for microscopic examination.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fluorescent mounting medium with dapi
1
EdU and Ki67 Immunofluorescence Assays
2
Immunohistochemical Analysis of IPO7 in Tissues
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Tissue sections were deparaffinized, dehydrared and then antigen retrieval was done in citric acid buffer (pH 6.0). The tissues were blocked with bovine serum albumin (BSA) for 1 hour before incubation overnight with the anti-IPO7 monoclonal antibody (1:100, sc365231, Santa Cruz) at 4 °C. The sections were then incubated with fluorescent secondary antibodies (1:200, A23210, Abbkine) for 1 hour. Then the tissues were washed 3 times in phosphate buffer saline (PBS), after which they were mounted in fluorescent mounting medium with DAPI (ZSGB-BIO). For cell immunofluorescence, the cells were fixed with 4% PFA at 4 °C, then permeabilized with 0.1% Triton X100 (BioFroxx), blocked by 3% BSA at 37 °C for 1 hour and were incubated with rabbit-anti-RUNX2 (YT5356, Immunoway) and mouse-anti-IPO7 (sc365231, Santa Cruz) antibodies overnight at 4 °C, then washed 3 times with PBS and incubated with fluorescent secondary antibodies for 1 hour (A23220, Abbkine; ANT034, Antgene). The fluorescence images were observed under fluorescence microscopy (Olympus 1 × 83, Japan).
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