HucMSCs (7530, ScienCell Research Laboratories, CA, USA) were subcultured to passage 4 in the medium (7501, ScienCell Research Laboratories).
The typical surface markers of hucMSCs were tested by ow cytometry. HucMSCs were resuspended in bovine serum albumin and combined with CD29 (1:100, 553715, BD Biosciences, CA, USA), CD34 (1:200, ab81289, Abcam, MA, USA), CD73 (1:50, 550257, BD Biosciences) and CD90 (1:100, 555595, BD Biosciences), respectively. HucMSCs were detected by ow cytometry after resuspending with phosphatebuffered saline (PBS).
HucMSCs were placed in osteogenic medium (0.1 mM dexamethasone, 10 mM β-glycerophosphate and 50 mM ascorbyl phosphate) or adipogenic medium (Cyagen Biosciences, CA, USA). Two weeks later, the osteogenic and adipogenic differentiation potentials were evaluated by Alizarin Red and Oil Red O staining [16, 17] .
Cell transfection miR-342-3p negative control (NC), miR-342-3p agomir and miR-342-3p antagomir (Sangon, Shanghai, China) were utilized to transfect hucMSCs using Lipofectamine 3000 (Invitrogen, CA, USA) [18] .
The typical surface markers of hucMSCs were tested by ow cytometry. HucMSCs were resuspended in bovine serum albumin and combined with CD29 (1:100, 553715, BD Biosciences, CA, USA), CD34 (1:200, ab81289, Abcam, MA, USA), CD73 (1:50, 550257, BD Biosciences) and CD90 (1:100, 555595, BD Biosciences), respectively. HucMSCs were detected by ow cytometry after resuspending with phosphatebuffered saline (PBS).
HucMSCs were placed in osteogenic medium (0.1 mM dexamethasone, 10 mM β-glycerophosphate and 50 mM ascorbyl phosphate) or adipogenic medium (Cyagen Biosciences, CA, USA). Two weeks later, the osteogenic and adipogenic differentiation potentials were evaluated by Alizarin Red and Oil Red O staining [16, 17] .
Cell transfection miR-342-3p negative control (NC), miR-342-3p agomir and miR-342-3p antagomir (Sangon, Shanghai, China) were utilized to transfect hucMSCs using Lipofectamine 3000 (Invitrogen, CA, USA) [18] .