Cell viability of ALL cell lines was quantified using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA), following the manufacturer’s instructions. Absorbance was measured at 440 nm using a Labsystem Multiskan EX (Thermo Fisher Scientific). Cell viability of primary leukemic ALL cells was analyzed using the RealTime-Glo™ MT Cell Viability Assay (Promega) following the manufacturer’s recommendations. Luminescence was measured using a Glomax microplate luminometer (Promega). Viability reduction was expressed as a percentage of the controls (normalized to 100%). The additive, synergistic, and antagonistic effect of the drug combinations was evaluated according to the Chou-Talalay equation (Chou 2010 ), using Compusyn Software (ComboSyn Incorporated, Paramus, NJ, USA). Based on developer instructions, we defined the following: synergism where CI < 1; additivity where CI = 1; and antagonism where CI > 1.
Labsystem multiskan ex
Manufactured by Thermo Fisher Scientific
The Labsystem Multiskan EX is a microplate reader designed for versatile absorbance-based measurements. It provides a reliable and efficient platform for various applications, including enzyme-linked immunosorbent assays (ELISAs), cell-based assays, and colorimetric assays. The instrument offers a wide range of wavelength options and can accommodate 6- to 384-well microplates.
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2 protocols using labsystem multiskan ex
1
Quantifying Cell Viability with Cytotoxicity Assays
2
Cell Viability Quantification and Drug Synergy
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Cell viability of ALL cell lines was quantified using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI, USA), following the manufacturer's instructions. Absorbance was measured at 440 nm using a Labsystem Multiskan EX (Thermo Fisher Scientific). Cell viability of primary leukemic ALL cells was analyzed using the RealTime-Glo™ MT Cell Viability Assay (Promega) following the manufacturer's recommendations. Luminescence was measured using a Glomax microplate luminometer (Promega). Viability reduction was expressed as a percentage of the controls (normalized to 100%). The additive, synergistic, and antagonistic effect of the drug combinations was evaluated according to the Chou-Talaley equation (Chou 2010) , using Compusyn Software (ComboSyn Incorporated, Paramus, NJ, USA). Based on developer instructions we defined: synergism where CI < 1; additivity where CI = 1; antagonism where CI > 1.
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