Mini protean 2 dual

In order to evaluate the integrity of nanohydrogel during the release experiments, a native-PAGE or "nondenaturing" gel electrophoresis, was performed. Native-PAGE analyses were carried out using the Mini-Protean II dual slab cell system equipped with a PAC 300 power supply (Bio-Rad Laboratories, Hercules, CA, USA). The resolving and stacking gel contained 10 and 4% of polyacrylamide, respectively. The gels were stained with 0.2% (w/v) silver nitrate (Chevallet et al., 2006) . Standard marker proteins PageRuler™ Broad Range Unstained Protein Ladder was used to identify samples by their molecular weight.

In order to evaluate the integrity of nanohydrogel during the digestion experiments, a native-PAGE or "nondenaturing" gel electrophoresis, was performed. Native-PAGE analyses were carried out using the Mini-Protean II dual slab cell system equipped with a PAC 300 power supply (Bio-Rad Laboratories, Hercules, CA, USA). The resolving and stacking gel contained 12.5 and 3.5% of polyacrylamide, respectively. The gels were stained with silver nitrate methodology (Chevallet, Luche, & Rabilloud, 2006) . Standard marker proteins PageRuler Unstained Broad Range Protein Ladder from Thermo Scientific was used to identify samples by their molecular weight.