Strength ringer s solution

The Serial dilutions were prepared using ¼ strength ringer's solution (Oxoid Limited, Basingstroke, Hampshire, England). Enumeration of Lactobacillus plantarum MNC21 was carried out by pour plating selected serial dilutions in MRS agar (CONDA, Madrid, Spain) and incubating at 30°C for 48 h. Enumeration of Saccharomyces cerevisiae MNC21Y was carried out by surface plating selected serial dilutions of the culture in Potato Dextrose Agar (CONDA, Madrid, Spain) with chloramphenical supplement and incubating at 30°C for 72 h. Microbial counts were determined at days 0, 5, 10, 20, 30, 40, 50, 60, 70, 80 and 90 during storage of starters at 5°C and only up to day 30 for starters stored at -18°C.

Serial dilutions were prepared using ¼ strength ringer's solution (Oxoid Limited, Basingstroke, Hampshire, England). Enumeration of Lactobacillus plantarum MNC21 was carried out by pour plating selected serial dilutions in MRS agar (CONDA, Madrid, Spain) and incubating at 30°C for 48 h. Enumeration of Saccharomyces cerevisiae MNC21Y was carried out by surface plating selected serial dilutions of the culture in Potato Dextrose Agar (CONDA, Madrid, Spain) with chloramphenical supplement and incubating at 30°C for 72 h. Microbial counts were determined at days 0, 5, 10, 20, 30, 40, 50, 60, 70, 80 and 90 during storage of starters at 5°C and only up to day 30 for starters stored at -18°C.