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Aqualab dew point hygrometer model 4 te

Manufactured by METER Group
Sourced in United States

The Aqualab dew point hygrometer model 4 TE is a laboratory instrument designed to accurately measure the water activity of various samples. It uses the chilled-mirror dew point technique to determine the equilibrium relative humidity of the sample, providing a precise and reliable measurement of the product's water activity.

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2 protocols using aqualab dew point hygrometer model 4 te

1

Physicochemical Characterization of Salad Dressings

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A Crison Basic 20+ pH meter (Crison S.A. Barcelona, Spain) was used to measure the pH of salad dressings. For the total titratable acidity (TTA) determinations, 10 g of each sample were mixed with 40 mL of distilled water and titrated with a 0.1 N NaOH solution until a pH value of 8.30. Total titratable acidity was expressed as g acetic acid/100 g of dressing. The water activity (aw) of samples was measured by an Aqualab dew point hygrometer model 4 TE (Decagon Devices, Inc., Washington, USA) at 25 ºC. The sodium chloride analysis was carried out by an automatic Chloride Analyser (Sherwood Scientific Ltd., Cambridge, UK) and the results were expressed as g NaCl/100 g of sample.
To study the stability of salad dressings against creaming, 5 g of each sample were transferred to a cylindrical glass container, sealed with a plastic cap and stored until analysed. The extent of creaming was calculated by employing Eq. ( 1): H% = (Ht/H0) x 100
(1)
where Ht represents the visible separation layer and H0 is the initial emulsion height.
Finally, the colour parameters (L*, a*, and b*) of salad dressings were measured by a spectrocolorimeter (CM-3600d, Minolta Co., Tokyo, Japan) with an observer 10º and illuminant D65. The Whiteness index (WI) was calculated by Eq. ( 2) and colour variations (2)
(3)
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2

Moisture, Salt, and Quality Assessment of Fish Samples

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Moisture contents (g/100 g) were determined in accordance with AOAC method 950.46 (1997) . Sodium chloride content in the liquid phase (Z NaCl (g NaCl/mL)) was measured in accordance with the procedure described by Fuentes, Fern andez-Segovia, Serra, and Barat (2010) . pH measurements were taken with a micropH 2001 digital pH-meter (Crison Instruments, S.A., Barcelona, Spain) and a puncture electrode (Crison 5231) at five different sample locations. Water activity (a w ) was measured in minced samples with a fast water activity-meter (Aqualab dew point hygrometer model 4 TE, Decagon Devices, Inc., Washington, USA). Total volatile basic nitrogen (TVB-N) and trimethylamine nitrogen (TMA-N) contents (mg N/100 g) were determined according to the method described by Malle and Tao (1987) . Weight changes in fish samples (DMt) were calculated according to Eq. ( 1).
where M t is the sample weight at time t (g) and M 0 is the initial sample weight (g) HPLC was used to determine the ATP-related compounds, consisting of inosine-50-monophosphate (IMP), inosine (Ino), and hypoxanthine (Hx), following the method described by Rizo et al. (2015b) . K 1 -value was calculated in accordance with Eq. ( 2):
where IMP is inosine 5 0 -monophosphate, Ino is inosine and Hx is hypoxanthine (mmol/g).
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