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Annexin 5 fluorescein isothiocyanate propidium iodide fitc pi kits

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Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) kits are used to detect and quantify apoptosis (programmed cell death) and necrosis in cells. The kits utilize Annexin V, a calcium-dependent phospholipid-binding protein, and propidium iodide, a fluorescent dye that binds to DNA, to distinguish between viable, apoptotic, and necrotic cells.

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Facscalibur, by BD (2 mentions)

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Annexin V-FITC/PI kit (53 citations) Annexin V-FITC/Propidium Iodide Kit (17 citations) Annexin V-fluorescein isothiocyanate (FITC) (12 citations) Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) kit (8 citations) Annexin V/propidium iodide (PI) kit (8 citations) Annexin V/propidium iodide (8 citations) Annexin-V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) (4 citations) Annexin V-fluorescein isothiocyanate (V-FITC)/propidium iodide (PI) kit (3 citations) Annexin V‐fluorescein isothiocyanate (FITC) kit (2 citations) Annexin V-fluorescein isothiocyanate kit (2 citations)

2 protocols using annexin 5 fluorescein isothiocyanate propidium iodide fitc pi kits

1

Cisplatin-Induced Apoptosis Assay

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After 24 h of transfection, the cells were treated with 1 μM cisplatin [32] for 72 h. An apoptosis assay was subsequently performed using Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) kits (KeyGEN Biotech. Co., Ltd., Nanjing, China). The cells were subsequently observed and analyzed using a flow cytometer (FACSCalibur, BD Biosciences, Franklin Lakes, NY, USA). The experiment was performed in triplicate, with the respective average values obtained and recorded.
Dong Y., Xu T., Fan L., Liu K., & Li G. (2020). microRNA-216b enhances cisplatin-induced apoptosis in osteosarcoma MG63 and SaOS-2 cells by binding to JMJD2C and regulating the HIF1α/HES1 signaling axis. Journal of Experimental & Clinical Cancer Research, 39(1).
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2

Cisplatin-Induced Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
After 24 h of transfection, the cells were treated with 1 μM cisplatin [32 (link)] for 72 h. An apoptosis assay was subsequently performed using Annexin V-fluorescein isothiocyanate/propidium iodide (FITC/PI) kits (KeyGEN Biotech. Co., Ltd., Nanjing, China). The cells were subsequently observed and analyzed using a flow cytometer (FACSCalibur, BD Biosciences, Franklin Lakes, NY, USA). The experiment was performed in triplicate, with the respective average values obtained and recorded.
Yang D., Xu T., Fan L., Liu K, & Li G. (2020). microRNA-216b enhances cisplatin-induced apoptosis in osteosarcoma MG63 and SaOS-2 cells by binding to JMJD2C and regulating the HIF1α/HES1 signaling axis. Journal of Experimental & Clinical Cancer Research : CR, 39, 201.
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