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4 protocols using iron iii chloride

1

Detailed Preparation of Enriched Compounds

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The 3D-enriched fragment library was purchased from Life Chemicals and divided into mixtures of 4–5 fragments as previously described [11 (link)]. Amino acids, uracil, thiamine-HCl, LB broth, biotin, and nicotinic acid were purchased from RPI Corp. The 5-fluoroindole, magnesium sulfate, succinic acid, calcium chloride, Iron(III) chloride, 15N ammonium chloride, L-Moses, and imidazole were purchased from Millipore Sigma. Potassium diphosphate, potassium monophosphate, sodium phosphate, manganese (II) chloride, zinc(I) chloride, and sodium chloride were purchased from Fischer Scientific. Cobalt (II) chloride, Ethylenediaminetetraacetic acid, and copper (II) chloride was purchased from Acros Organics. Boric acid was purchased from Mallinckrodt. Deuterium oxide and dimethyl sulfoxide-d6 were purchased from Cambridge Isotope Laboratories. GSK4027 was purchased from Cayman Chemicals and TP238 was synthesized previously [55 (link)].
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2

Photochemical Silver Nanoparticle Synthesis

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Silver nitrate (AgNO3, 99%, lot #22C0256162), sulfuric acid (lot #2020081109), mEthanol (lot #0000265583), chloroform (lot #21J1456829), isopropanol (lot #22K1561065), and hexane (lot #0000258628) were purchased from VWR. Ethanol (Supelco, 200 proof, lot #62154), Dragendorff reagent (lot #BCBX4809), trisodium citrate dihydrate (lot #SLCP1502), copper(ii) sulphate (lot #MKCR0607), hydrochloric acid (lot #MKCN0724), and iron(iii) chloride (lot #STBK8949) were purchased from Millipore Sigma. Sodium copper chlorophyllin (lot #AD-22021) was purchased from Ward's Science. Acetone was purchased from Fisher Scientific. Potassium carbonate (lot #50019773) was purchased from BeanTown Chemical. Mg chips (lot #35695600) were purchased from Stream Chemicals. Water (H2O, 18.2 MΩ cm) was obtained from a water purification system (Sartorius, Arium mini).
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3

Comprehensive Characterization of Reagents

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All reagents used for characterization were analytical grade. Sulfuric acid, phenol, and iron sulfate were supplied by Merck (Darmstadt, Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tris (2-pyridyl)-S-triazine (TPTZ), potassium hydroxide, potassium chloride, iron (III) chloride, sodium chloride, potassium dihydrogen phosphate, sodium phosphate, sodium azide, phloroglucinol, and sodium acetate were from Millipore Sigma (St. Louis, MO, USA). 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sodium carbonate, Folin–Ciocalteau reagent, chlorohydric acid, glucose, Trolox, sodium hydroxide, and methanol were from Panreac (Barcelona, Spain).
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4

Hydroponic Lettuce Cultivation with Automated Monitoring

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Anhydrous d-glucose was purchased from Acros Organics (Geel, Belgium). Original formula Windex® (S.C. Johnson, Wisconsin, USA) and a 20 L microwave (Hamilton Beach, 1000 W) were purchased from a local supplier. Britton Robinson buffer, phosphate-buffered saline (PBS), HEPES buffer, NaOH, mercury(i) nitrate, and nitric acid were purchased from Fisher Scientific (Thermo Fisher Scientific, Massachusetts, USA). Fluorescein, iron(iii) chloride, and all other salts used for interferent testing were purchased from Millipore Sigma (Darmstadt, Germany). A Hydrofarm Root Spa 8 DWC hydroponic system (HydroFarm, California, USA), 250 W full spectrum Pro Series P2500 LED light (ViparSpectra, California, USA), EC meter (HoneForest Model YL-TDS2-A, United States), pH meter, and hydroponic nutrient solutions with chelated iron (Fe-EDDHA) were purchased from an online supplier. Lettuce seeds, rockwool germination cubes, and clay pebble growing media were purchased from a local nursery supplier.
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