Annexin 5 apc solution

Manufactured by Keygen Biotech

Sourced in China

Annexin V-APC solution is a laboratory reagent used for the detection and quantification of apoptotic cells. Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine, which is exposed on the surface of apoptotic cells. The APC (Allophycocyanin) fluorescent label allows for the visualization and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Facs caliber instrument, by BD (1 mentions) Facs caliber, by BD (1 mentions) Flow cytometry, by BD (1 mentions)

Close spelling variants of this product

Annexin V-APC (40 citations) Annexin V (31 citations) Annexin V-APC (APC) (2 citations)

3 protocols using annexin 5 apc solution

Apoptosis Analysis of Huh-7 Cells

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After transfection with sh-Ctrl or sh-CDCA5 lentiviruses for 72 h, cells were stained with Annexin V-APC solution (KeyGEN, Jiangsu, China) according to the manufacturer’s instructions. Numbers of apoptotic Huh-7 cells were then determined using flow cytometry with a fluorescence-activated cell sorting (FACS) caliber instrument (BD). Percentages of apoptotic cells were calculated according to Annexin V staining.

Shen Z., Yu X., Zheng Y., Lai X., Li J., Hong Y., Zhang H., Chen C., Su Z, & Guo R. (2018). CDCA5 regulates proliferation in hepatocellular carcinoma and has potential as a negative prognostic marker. OncoTargets and therapy, 11, 891-901.

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Annexin V Apoptosis Assay by Flow

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Cells were incubated with Annexin V-APC solution (KeyGEN) for 15 min. The percentage of apoptotic was analyzed by flow cytometry (FACS Caliber, Becton-Dickinson).

Shen A., Liu L., Chen H., Qi F., Huang Y., Lin J., Sferra T.J., Sankararaman S., Wei L., Chu J., Chen Y, & Peng J. (2019). Cell division cycle associated 5 promotes colorectal cancer progression by activating the ERK signaling pathway. Oncogenesis, 8(3), 19.

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Apoptosis Quantification by Flow Cytometry

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The cells stably transduced with the lentivirus vector were seeded in 6-well plates. After culture for 72 h, cells were incubated with Annexin V-APC solution (KeyGEN, Jiangsu, China) for 20 min according to the manufacturer's instructions. The percentage of apoptotic cells were analyzed by flow cytometry (Becton Dickinson). The experiments were performed in triplicate.

Shen Z., Chen Y., Li L., Liu L., Peng M., Chen X., Wu X., Sferra T.J., Wu M., Lin X., Cheng Y., Chu J., Shen A, & Peng J. (2020). Transcription Factor EBF1 Over-Expression Suppresses Tumor Growth in vivo and in vitro via Modulation of the PNO1/p53 Pathway in Colorectal Cancer. Frontiers in Oncology, 10, 1035.

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