Monovalent 2009 h1n1 influenza pdm09 vaccine nr 20083

OVA (A5503) and MPL (L6895) were purchased from Sigma (St. Louis, MO). AddaVax, Pepinh-MYD, and Pepinh-Control peptides were purchased from Invivogen (San Diego, CA). CpG 1826 was synthesized by Trilink Biotechnologies (San Diego, CA). Alum (Imject) was ordered from Thermo Fisher Scientific (Rockford, lL). rHA of Influenza A/California/07/2009 (H1N1) (FR559) was obtained from International Reagent Resource (IRR, Manassas, VA). Monovalent 2009 H1N1 influenza (pdm09) vaccine (NR-20083) was obtained from BEI Resources (Manassas, VA). Fluorescence-conjugated antibodies used in immunostaining and flow cytometry were purchased from eBiosciences, Biolegends, or Affymetrix, all from San Diego, CA.

Recombinant HA (rHA) of Influenza A/California/07/2009 (H1N1) (FR-559) was obtained from International Reagent Resource (IRR, Manassas, VA, USA). Monovalent 2009 H1N1 influenza (pdm09) vaccine (NR-20083) was obtained from BEI Resources (Manassas, VA, USA). Fluorescence-conjugated antibodies used in immunostaining and flow cytometry were purchased from Biolegends (San Diego, CA, USA). Horseradish peroxidase (HRP)-conjugated anti-mouse IgG secondary antibodies (45000679) was obtained from Fisher Scientific (Waltham, MA, USA). CpG 1018 was synthesized by Trilink Biotechnologies (San Diego, CA, USA). Chicken red blood cells (RBCs) were obtained from Charles River (Wilmington, MA, USA).