Stata version 14.2 statistical software

Variables measured in the interval scale are summarized as means and standard deviations, whereas variables recorded in the ordinal or nominal scale are summarized as counts and percentages. The chi-square test was used to test the frequencies of variables in the ordinal or nominal scales. Maternal associations with offspring %FM were first evaluated using the entire cohort (n=209). Then, a secondary analysis was conducted in the subset of participants (n=136) for whom maternal-father-offspring data were available.
The association between infant’s %FM at two weeks of age and parental/infant characteristic was assessed using linear regression, whereas multiple linear regression was used to model infant’s %FM at two weeks of age and all characteristics simultaneously. The most parsimonious linear regression model was constructed using least absolute shrinkage and selection operator (LASSO) linear regression, although the same model resulted using step-wise linear regression. Data management and analysis was performed using the Stata version 14.2 statistical software (Stata Corp., College Station Texas).

Variables measured in the interval scale are summarized as means and standard deviations, whereas variables recorded in the ordinal or nominal scale are summarized as counts and percentages. The association between offspring’s %FM during the first two years of life and each maternal/infant characteristic was assessed using regressions, whereas mixed models were used to model offspring %FM during the first two years of life and all characteristics simultaneously using a time-varying covariate approach when measures were available at each trimester (e.g. REE, cytokines). The most parsimonious model was constructed using least absolute shrinkage and selection operator (LASSO) linear regression. In light of the sexual dimorphism observed in animal models and clinical studies in response to maternal obesity (6 (link),19 (link)) models were further build independently for male (N=128) and female (N=96). Data management and analysis was performed using the Stata version 14.2 statistical software (Stata Corp., College Station Texas) and R version 3.6.1, whereas the recursive partitioning was performed using JMP Pro version 13.0 statistical software (SAS Institute Inc., Cary, NC).