Hcclm3

Human hepatoma cell line Huh7 was purchased from Procell (Wuhan, China). Human hepatoma cell line Hep3B2.1-7 was purchased from Beyotime Biotechnology (Beijing, China). Human hepatocellular carcinoma cell line HepG2 was purchased from American Type Culture Collection (ATCC, Rockville, MD, USA). Human hepatocarcinoma cell line HCCLM3 was obtained from the China Center for Type Culture Collection (CCTCC, Wuhan, China). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific) with 10% fetal bovine serum (FBS, Hyclone, Logan, UT, USA) at 37°C in a 5% CO₂ humidified incubator.

The human hepatocellular carcinoma cell line HCC-LM3 and mouse hepatocellular carcinoma cell line H22 were obtained from the China Center for Type Culture Collection (Shanghai, China), authenticated by short tandem repeat (STR) analysis, and tested for mycoplasma contamination. The mouse hepatocellular carcinoma cell line Hepa1-6, human embryonic kidney cell line 293T, human T-cell leukemia cell line Jurkat, human chronic myeloid leukemia cell line K562, human alveolar adenocarcinoma cell line A549, mouse melanoma cell line B16-F10 and colon carcinoma cell line CT26.WT were obtained from the American Type Culture Collection (Manassas, VA, United States). 293T-VSV-G, an engineered 293T cell line that expresses VSV-G under control of the cytomegalovirus (CMV) early enhancer/chicken beta-actin (CAG) promoter, was engineered by our team by transducing cells with a lentiviral vector. Jurkat K562 and H22 cells were cultured in Roswell Park Memorial Institute 1640 (RPMI 1640) medium and other cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 units/ml penicillin, and 0.1 mg/ml streptomycin (all from Thermo Fisher Scientific, Gibco, Grand Island, NY, United States). All cells were maintained in a humidified incubator with an atmosphere containing 5% CO₂ at 37°C.

HepG2, Huh7, Hep3B, Hepa1-6, H22, 293FT, and HEK 293 T cells were purchased from Cell Resource Center, Peking Union Medical College. HCCLM3 cells were purchased from the China Center for Type Culture Collection (CCTCC). The cells had been authenticated by short tandem repeat (STR) profiling and characterized by mycoplasma detection and cell viability detection. HepG2 cells were cultured and maintained in MEM (Invitrogen, 11090081) supplemented with 10% fetal bovine serum (FBS; Invitrogen, CA, USA) and nonessential amino acids (Invitrogen, 11140050) under 5% carbon dioxide. HCCLM3, Huh7, Hepa1-6 and HEK 293 T cells were cultured and maintained in DMEM supplemented with 10% FBS. Hep3B cells were cultured and maintained in MEM-EBSS supplemented with 10% FBS. H22 cells were cultured and maintained in RPMI 1640 medium supplemented with 10% FBS. All cell lines were verified negative for mycoplasma contamination by MycoAlert^TM Mycoplasma Detection Kit (Lonza, LT07-318).