Cholestest tg

Hepatic triglyceride and cholesterol levels were measured at Skylight Biotech (Akita, Japan) using the Folch technique with Cholestest TG and Cholestest CHO kits (Sekisui Medical, Tokyo, Japan), respectively.

Hepatic TG and TCHO content were measured at Skylight Biotech (Akita, Japan) using the Folch method [27 (link)] with Cholestest TG and Cholestest CHO kits (Sekisui Medical, Tokyo, Japan), respectively. The values were standardized to 1 g of liver weight.

Huh-7 cells (1.5 × 10⁵ cells/well) and primary mouse hepatocytes (1 × 10⁵ cells/well) were seeded in 6- and 24-well plates and prepared for lipid extraction at days 4 and 7 of transfection, respectively. Cells were homogenized in the lysis buffer (1% Triton X-100/150 mM NaCl/1 mM EDTA/50 mM Tris–HCl, pH 7.5) and lipids were extracted according to the Folch method^{94 (link)}. Briefly, 4 volume of chloroform (Fujifilm)/methanol (Nacalai Tesque) (1:2) was added to the homogenates and mixed well. After adding 1.25 volumes each of chloroform and distilled water and mixing, the lower phase was separated by centrifugation. The collected lower phase was dried-up and the residual lipids were resolved in 2-propanol (Nacalai tesque). The TG levels were measured using the enzymatic assay kit Cholestest TG (Sekisui Medical, Tokyo, Japan). TG values were normalized by the protein concentration of the homogenates.