Lipopolysaccharide from Escherichia coli 0111:B4 was purchased from Sigma-Aldrich (St. Louis, MO, United States). Recombinant mouse IFN-γ protein was purchased from R&D systems. The bacterially expressed recombinant mouse C8G protein was prepared as described previously (Lee et al., 2008 (link)). Briefly, the recombinant mouse C8G protein without the signal peptide was expressed in E. coli (strain BL21) as a glutathione S-transferase (GST) fusion protein. The protein was purified using glutathione Sepharose 4B beads (GE Healthcare, Chicago, IL, United States). GST was removed by thrombin digestion. C8G was purified and concentrated. SDS-PAGE with Coomassie Brilliant Blue R250 staining showed that the purity of C8G was over 95%. JTE013, S1P, STATTIC, and BAY 11-7082 were purchased from Cayman Chemical (Ann Arbor, MI, United States). CYM-5478 was purchased from Aobious Inc. (Gloucester, MA, United States).
Cym 5478
Manufactured by Aobious
Sourced in United States
The CYM-5478 is a laboratory equipment designed for general scientific applications. It serves as a versatile tool for various experimental procedures. The core function of the CYM-5478 is to provide a controlled and consistent environment for conducting laboratory experiments.
Automatically generated - may contain errors
Lab products found in correlation
Sphingosine 1 phosphate (s1p), by Cayman Chemical (1 mentions)
Glutathione sepharose 4b beads, by GE Healthcare (1 mentions)
Jte 013, by Cayman Chemical (1 mentions)
Lipopolysaccharide from escherichia coli 0111 b4, by Merck Group (1 mentions)
Stattic, by Cayman Chemical (1 mentions)
Bay 11 7082, by Cayman Chemical (1 mentions)
2 protocols using cym 5478
1
Recombinant Mouse C8G Protein Purification
2
Modulating S1pr2 for Fin Fold Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
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S1pr2 selective modulatory agent, CYM-5478 (Aobious), was dissolved in DMSO as 25mM stock solution and added to embryos from 3hpf to 48hpf at final concentration of 10-50µM, and then scored for fin fold abnormalities.
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