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Dipstick assay kit

Manufactured by Abcam
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Sourced in United States

The Dipstick Assay kit is a laboratory equipment designed for rapid qualitative or semi-quantitative detection of target analytes in a sample. It utilizes a paper-based test strip with reagents that produce a visible color change upon interaction with the analyte, allowing for quick and easy interpretation of results.

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Lab products found in correlation

Close spelling variants of this product

Complex I Enzyme Activity Dipstick Assay Kit Frataxin Protein Quantity Dipstick Assay Kit )/Complex IV (ab109876) Enzyme Activity Dipstick Assay Kit PDH) Enzyme Activity Dipstick Assay Kit MitoProfile Dipstick Assay Kit

2 protocols using dipstick assay kit

1

Mitochondrial Complex I Activity Assay

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Complex I activity was determined using the Dipstick Assay kit (MitoSciences, Eugene, OR, USA) according to the manufacturer's protocols, as previously described [26 (link)].
Zhou X., Chen J., Yi G., Deng M., Liu H., Liang M., Shi B., Fu X., Chen Y., Chen L., He Z., Wang J, & Liu J. (2015). Metformin suppresses hypoxia-induced stabilization of HIF-1α through reprogramming of oxygen metabolism in hepatocellular carcinoma. Oncotarget, 7(1), 873-884.
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2

Assaying Cytochrome C Oxidase Activity

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The Complex IV human enzyme activity Dipstick Assay Kit (ab109876; MitoSciences) was used to assess cytochrome C oxidase activity in control and PISD fibroblasts according to the manufacturer's instructions. The assay was performed using 50 μg lysates. Following signal development, dipsticks were imaged on an Amersham Imager (AI600) and signal intensity, corresponding with complex IV activity, was quantified using ImageJ software (Schindelin et al, 2012 (link)). Values were determined from the average of at least three technical replicates from three independent biological replicates.
Zhao T., Goedhart C.M., Sam P.N., Sabouny R., Lingrell S., Cornish A.J., Lamont R.E., Bernier F.P., Sinasac D., Parboosingh J.S., Vance J.E., Claypool S.M., Innes A.M, & Shutt T.E. (2019). PISD is a mitochondrial disease gene causing skeletal dysplasia, cataracts, and white matter changes. Life Science Alliance, 2(2), e201900353.
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