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Sh sy5y cells crl 2266

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SH-SY5Y cells (CRL-2266™) are a human-derived neuroblastoma cell line. They are adherent cells derived from a bone marrow biopsy sample. The cells can be used for research purposes.

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2 protocols using sh sy5y cells crl 2266

1

Cell Culture Protocols for Cancer and Neuroblastoma

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Human breast cancer MDA-MB-231 cells (ATCC® HTB-26™), acute promyelocytic leukemia HL-60 cells (ATCC® CCL-240™), neuroblastoma SH-SY5Y cells (CRL-2266™) and murine neuroblastoma Neuro-2a cells (CCL-131™) were obtained from the American Type Culture Collection (Manassas, VA, USA). The MDA-MB-231 and HL-60 cells were cultured in complete DMEM/10% FBS and RPMI 1640/10% FBS, respectively, containing 1% of penicillin-streptomycin solution. The SH-SY5Y cells were cultured in MEM containing 10% FBS and 1% penicillin-streptomycin solution. The Neuro-2a cells were cultured in DMEM containing 10% FBS and 1% penicillin-streptomycin solution. The cell cultures were incubated at 37 °C in a humidified atmosphere containing 5% (v/v) CO2.
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2

Culturing Human Neuroblastoma SH-SY5Y Cells

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The human neuroblastoma SH-SY5Y cells (CRL-2266) obtained from American Type Culture Collection (Manassas, VA, USA) were cultured in the RPMI 1640 medium (Gibco, Invitrogen, Carlsbad, CA, USA) containing 8% FBS (Gibco, Invitrogen, USA), 5% penicillin and streptomycin (Gibco, Invitrogen, USA). At an initial plating density of 0.5 million cells per plate, cells were routinely trypsinized and subcultured.
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