Ivis lumina xr series 3

All animal experiments were performed according to the Guidelines for Use and Care of Animals at Xi’an Jiaotong University (No. 2019–293). The Department of Experimental Animals, Health Science Center, Xi’an Jiaotong University supplied male nude mice (8-week-old). To establish a subcutaneous gastric tumor model, 1 × 10⁷ BGC-823 cells suspended in 200 μL of saline/Matrigel were inoculated subcutaneously at the left flank of the male nude mice. Furthermore, to establish an orthotopic gastric tumor model, we maintained the nude mice under anesthesia and opened the abdomen of the nude mice, and then injected 1 × 10⁷ BGC-823 cells suspended in 100 μL of saline/Matrigel directly between the submucosa and the muscular layer of the stomach, finally sutured the wound according to the previous method (Kang et al., 2021 (link)). When the long axis of the tumor diameter arrived at 1–2 cm or palpation of abdominal nodules, MB (250 µM/100 µL) in saline containing 10% BSA was injected into tumor-bearing mice through the tail vein. In brief, isoflurane was used to anesthetize the tumor-bearing mice with the image of 0.5, 1, 2, 4, 8, 24, 48, and 72 h under NIR imaging (PerkinElmer IVIS Lumina XR Series III, United States). After finishing the real-time imaging, we euthanized mice and dissected major organs and gastric tumor tissues for fluorescence imaging. Each sample used three mice.