6220 mass spectrometer

IR and UV spectra were measured on MIDAC M-series FTIR and Shimadzu UV-1201 spectrophotometers, respectively. ¹H and ¹³C NMR spectra were recorded on a Bruker Avance 500 spectrometer in CDCl₃, with CDCl₃ as reference. Mass spectra were obtained on an Agilent 6220 mass spectrometer. Open column chromatography was performed using Sephadex LH-20 and silica gel (230-400 mesh, Silicycle Co. USA). Semi-preparative HPLC was performed using Shimadzu LC-10AT pumps coupled with a semipreparative Phenomenex C-18 column (5 μm, 250 × 10 mm), a Shimadzu SPD M10A diode array detector, and a SCL-10A system controller. All isolated compounds were purified to 95% purity or better, as judged by HPLC (both UV and ELSD detection) before determining bioactivity.

IR and UV spectra were measured on MIDAC M-series FTIR and Shimadzu
UV-1201 spectrophotometers, respectively. ¹H and ¹³C NMR
spectra were recorded on a Bruker Avance 500 spectrometer in CDCl₃,
with CDCl₃ as reference. Mass spectra were obtained on an Agilent
6220 mass spectrometer. Open column chromatography was performed using Sephadex
LH-20. Solid phase extract was performed using silica gel (230-400 mesh,
Silicycle Co. USA). Semi-preparative HPLC was performed using Shimadzu LC-10AT
pumps coupled with a semipreparative Phenomenex C-18 column (5 μm, 250
× 10 mm), a Shimadzu SPD M10A diode array detector, and a SCL-10A system
controller.

1D and 2D NMR spectra were recorded on a Bruker Avance 500 spectrometer in CDCl3, with residual CHCl3 as internal reference. The high resolution ESI mass spectrum was obtained on an Agilent 6220 mass spectrometer.