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Biopet

Manufactured by Bioscan
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BioPET is a high-performance liquid chromatography (HPLC) instrument designed for the analysis and purification of biomolecules. It features a compact and modular design, providing users with a versatile and efficient tool for various applications in the life sciences and biotechnology industries.

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Asipro software, by Siemens (1 mentions)

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BIOPET 105 BioPET/CT

3 protocols using biopet

1

Tracking Bone Marrow Cell Dynamics in Fracture Healing

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One group of mice received 89Zr-oxine-labeled BM cell administration followed 1 day later by tibial injury (d1-fracture group). MicroPET/CT imaging (BioPET, Bioscan, Washington, DC) was performed 1 day after the cell transfer, before and after the injury, and on days 2 and 5. Another group of mice received 89Zr-oxine-labeled BM cells immediately after the tibial injury (d0-fracture group) followed by microPET/CT imaging on days 0, 1, 2, and 5. A 5-min emission PET scan per bed position was performed for a total of two bed positions on day 0, using a 400–700 keV energy window. On days 1, 2, and 5, scan time per bed position was increased to 6, 7.5, and 12.5 min, respectively, to account for radioactive decay. The acquired images were reconstructed using a three-dimensional ordered-subsets expectation maximization algorithm. VivoQuant software (inviCRO LLC, Boston, MA) was used to fuse the maximum intensity projection PET images with CT images.
Asiedu K.O., Ferdousi M., Ton P.T., Adler S.S., Choyke P.L, & Sato N. (2018). Bone marrow cell homing to sites of acute tibial fracture: 89Zr-oxine cell labeling with positron emission tomographic imaging in a mouse model. EJNMMI Research, 8, 109.
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2

PSMA PET Imaging of Prostate Tumors

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Prior to imaging, all mice were anesthetized using isoflurane/O2 (1.5 % to 3 % v/v) and imaged (BioPET, Bioscan) at 1 h post [18F]DCFPyL injection (100–150 μCi; iv). Whole body images were acquired (2 bed positions; 5 min each) and reconstructed using three-dimensional ordered-subset expectation maximums (3D-OSEM) [27 (link)]. Mice in both the groups were imaged before initiation of ADT treatment (baseline) and at days 2, 7, 14, and 21 following treatment. Tumors were measured weekly (concurrent with PET imaging) by caliper, and the volume was calculated using the ellipsoid formula.
PET images were analyzed (ASIPro software, Siemens) by drawing regions of interest (ROI’s) for LuCaP73, LuCaP136, and LuCaP167 which encompassed the whole tumor volume. Additionally, ROIs were drawn for the thigh muscle, from which tumor:muscle PSMA PET uptake ratios (T:M) were calculated for each mouse. Statistical significance (P < 0.05) between the control and treated groups was determined using the student t-test.
Roy J., White M.E., Basuli F., Opina A.C., Wong K., Riba M., Ton A.T., Zhang X., Jansson K.H., Edmondson E., Butcher D., Lin F.I., Choyke P.L., Kelly K, & Jagoda E.M. (2021). Monitoring PSMA Responses to ADT in Prostate Cancer Patient-Derived Xenograft Mouse Models Using [18F]DCFPyL PET Imaging. Molecular imaging and biology, 23(5), 745-755.
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3

Whole-body PET Imaging of Anti-PD-L1 in Mice

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Tumor-bearing mice were anesthetized with isoflurane/O2 (1.5%-3% v/v) and imaged at various times after intravenous injection of [89Zr]Zr-DFO-PD-L1 mAb (2.6-3.7 MBq [70-100 µCi], 80-110 pmol). Whole-body static PET images were obtained at 2 bed positions (FOV = 2.0 cm, total imaging time: 10 minutes) followed by CT images (2 bed positions, 10 minutes) using the BioPET (Bioscan Inc., Washington, District of Columbia). The images were reconstructed by a 3-dimensional ordered subsets expectation maximum (3D-OSEM).
Jagoda E.M., Vasalatiy O., Basuli F., Opina A.C., Williams M.R., Wong K., Lane K.C., Adler S., Ton A.T., Szajek L.P., Xu B., Butcher D., Edmondson E.F., Swenson R.E., Greiner J., Gulley J., Eary J, & Choyke P.L. (2019). Immuno-PET Imaging of the Programmed Cell Death-1 Ligand (PD-L1) Using a Zirconium-89 Labeled Therapeutic Antibody, Avelumab. Molecular Imaging, 18, 1536012119829986.
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