The HCC cell lines HepG2, HepG3B and SMMC7221 we used were obtained from the American Type Culture Collection (Rockville, MD, USA) in 2012 and authenticated using short tandem repeat (STR) analysis by Genewiz Inc. in 2014. STR analysis showed that the submitted samples were in good agreement with the reference cell lines. The HCC cell line Bel7402 was obtained from KeyGEN BioTECH and we finished our studies within 6 months. HepG2 cells were cultured in Modified Eagle's Medium supplemented with 10% foetal bovine serum, SMMC7721 cells were cultured in Dulbecco's Modified Eagle's Medium supplemented with 10% foetal bovine serum, while Bel7402 and HepG3B were cultured in Roswell Park Memorial Institute 1640 supplemented with 10% foetal bovine serum (Invitrogen). The vectors were transfected into cells using a percutaneous ethanol injection (cat no. 23966; Polysciences, Inc.).
Hepg3b
Manufactured by Thermo Fisher Scientific
Sourced in United States
The HepG3B is a cell line derived from human hepatocellular carcinoma. It is commonly used in cell biology research and drug discovery applications.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Thermo Fisher Scientific (2 mentions)
Hepg2, by American Type Culture Collection (2 mentions)
Hepg3b, by American Type Culture Collection (2 mentions)
Mhcc97h, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Smmc 7721, by Thermo Fisher Scientific (1 mentions)
Bel 7402, by Thermo Fisher Scientific (1 mentions)
Bel 7402, by Keygen Biotech (1 mentions)
Smmc 7221, by American Type Culture Collection (1 mentions)
Snu449, by Thermo Fisher Scientific (1 mentions)
Snu 449, by American Type Culture Collection (1 mentions)
2 protocols using hepg3b
1
HCC Cell Lines Characterization and Transfection
2
Molecular Mechanisms of Liver Cancer
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Human liver cancer cell lines SNU449, Hepg3b, and Hepg2 were purchased from the American Type Culture Collection (ATCC, USA), and MHCC‐97H cell line was obtained from the Liver Cancer Institute (Fudan University, Shanghai, China). SNU449 cells were incubated in ATCC‐formulated RPMI‐1640 Medium with 10% fetal bovine serum (FBS); in addition, ATCC‐formulated Eagle's Minimum Essential Medium containing 10% FBS was used to culture Hepg3b and Hepg2 cells. Dulbecco's modification of Eagle's medium (DMEM) (Thermo Fisher Scientific, USA) with 10% FBS was used to culture MHCC‐97H cells.
The overexpression vector of ASPM (pcDNA‐ASPM), shRNAs‐against ASPM, and METTL3 was purchased and designed by GeneChem Corporation (Shanghai, China) (TableS1 ). SNU449, Hepg3b, MHCC‐97H, and Hepg2 cells were transfected with indicated plasmids consistence with references of lipofectamine (Vision 2000, 11668‐019, Invitrogen, USA) and were cultured for 48 h.
The overexpression vector of ASPM (pcDNA‐ASPM), shRNAs‐against ASPM, and METTL3 was purchased and designed by GeneChem Corporation (Shanghai, China) (Table
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