Observation with SBF-SEM and analyses of acquired data were performed as described previously with slight modifications (53 (link), 54 (link)). SBF-SEM of the neuroblast chains was performed using a Merlin scanning electron microscope (Carl Zeiss) equipped with a 3View in-chamber ultramicrotome system (Gatan, Pleasanton, CA, USA). Sequential images were processed using FIJI. Segmentation of the cell membrane was performed using Microscopy Image Browser (55 (link)). 3D reconstruction was performed using Amira software (Visualization Sciences Group). For details, see the Supplementary Materials.
3view in chamber ultramicrotome system
Manufactured by Ametek
Sourced in United States
The 3View in-chamber ultramicrotome system is a laboratory equipment designed for high-resolution three-dimensional imaging of samples. It provides a controlled environment for sectioning and imaging of specimens at the nanometer scale.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 3view in chamber ultramicrotome system
1
3D Reconstruction of Neuroblast Chains
2
3D-EM Analysis of Myelinated Axons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Myelinated and demyelinated corpora callosa (i.e., 3 wks cuprizone) were analyzed by 3D EM as published by Ohno and colleagues [53 (link)]. To this end, the mice were perfused via the left ventricle with 2.5% (wt/vol) glutaraldehyde and 4% paraformaldehyde. The brains were subsequently removed, stained with heavy metals, and embedded in resin. 3D-electron microscopy (Serial block-face scanning EM) was performed by using a SigmaVP scanning electron microscope (Carl Zeiss) equipped with a 3View in-chamber ultramicrotome system (Gatan). Serial image sequences were generated at 80 nm-nm steps, providing 204.54 μm × 61.36 μm-wide image stacks at a resolution of 0.001 µm per pixel. The images were processed and measured with the open source program, Reconstruct (BU, Boston, MA, USA). The focus of this analysis was to detect mitochondria within axonal swellings.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!