Temozolomide tmz

Manufactured by Interchim

Sourced in France

Temozolomide (TMZ) is a compound used in laboratory research applications. It is a synthetic alkylating agent that can be used in the study of various biological and biochemical processes. TMZ is a white to off-white crystalline powder that is soluble in dimethyl sulfoxide, methanol, and ethanol. The core function of TMZ is to serve as a research tool for scientific investigations, without further interpretation or extrapolation on its intended use.

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Lab products found in correlation

Cell culture products, by Thermo Fisher Scientific (1 mentions) Mcl 1, by Santa Cruz Biotechnology (1 mentions) Pdest12, by Thermo Fisher Scientific (1 mentions) β tubulin, by Merck Group (1 mentions)

2 protocols using temozolomide tmz

Temozolomide Drug Preparation Protocol

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Temozolomide (TMZ) was from Interchim (Montluçon, France), all other drugs were from Sigma (Saint Louis, MO) unless otherwise noted. All cell culture products were obtained from Life Technologies (Carlsbad, CA).

Rabé M., Dumont S., Álvarez-Arenas A., Janati H., Belmonte-Beitia J., Calvo G.F., Thibault-Carpentier C., Séry Q., Chauvin C., Joalland N., Briand F., Blandin S., Scotet E., Pecqueur C., Clairambault J., Oliver L., Perez-Garcia V., Nadaradjane A., Cartron P.F., Gratas C, & Vallette F.M. (2020). Identification of a transient state during the acquisition of temozolomide resistance in glioblastoma. Cell Death & Disease, 11(1), 19.

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Temozolomide-Induced Apoptosis and Autophagy

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All reagents were from Sigma except for the Temozolomide (TMZ) obtained from Interchim (Paris, France). The stock solution of TMZ in DMSO at 100mM. Aliquots were frozen at −20°C and dilutions to the appropriate concentrations were done in culture medium just before the experiment. The following antibodies were used: Mcl-1 (sc-819 Santa Cruz), Bax (2D2, Sigma), Bak (556396- BD-Pharmingen USA), SQSTM1/p62(5114-Cell Signaling) and LC3B (2775- Cell Signaling), β Tubulin (T0198-Sigma), αActin (clone C4, MAB1501, Millipore). Secondary mouse and rabbit antibodies were from Jackson ImmunoResearch (UK). For MGMT transfection the plasmid pDEST 12.2 was obtained from In Vitrogen, Life technologies (CA) and the MGMT cDNA from ATCC, clone MGC-5186.

Gratas C., Séry Q., Rabé M., Oliver L, & Vallette F.M. (2014). Bak and Mcl-1 are essential for Temozolomide induced cell death in human glioma. Oncotarget, 5(9), 2428-2435.

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