Nucleolin 4e2

Manufactured by Abcam

Nucleolin (4E2) is a protein marker used in research applications. It is a nuclear phosphoprotein involved in the organization of nucleolar chromatin and in the process of ribosome assembly.

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Lab products found in correlation

γh2ax, by Merck Group (1 mentions) Phospho mst1 thr183 mst2 thr180, by Cell Signaling Technology (1 mentions) Phospho histone h2b ser14, by Merck Group (1 mentions) Phospho histone h2b ser14, by Cell Signaling Technology (1 mentions) Ubf f 9, by Santa Cruz Biotechnology (1 mentions) Lamin a c, by Cell Signaling Technology (1 mentions) Biorevo bz 9000, by Keyence (1 mentions) Vvl fitc, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Nucleolin (21 citations)

2 protocols using nucleolin 4e2

Comprehensive Histone Protein Analysis

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MST2 (Abcam, ab52641), phospho‐histone H2B (Ser14) (Cell Signalling, 6959), phospho‐histone H2B (Ser14) (Millipore, 07‐191), MST1 (Millipore, 07‐061), RASSF1A (3F3, Santa Cruz sc‐58470), UBF (F9, Santa Cruz, sc‐13125), SAV‐1 (Atlas, HPA001808), V5 (Cell Signalling, 13202), H2B (abcam, ab52484), H2A (Abcam, ab18255), H3 (96C10, Cell Signalling, 3638), H4 (Cell Signalling, 2592), γH2AX (JBW301, Millipore, 16‐193), nucleolin (4E2, Abcam, ab13541), lamin A/C (Cell signalling, 4777), α‐tubulin (B3, Sigma, T9822), RCC1 (Cell Signalling, 5134), phospho‐MST1 (Thr183)/MST2(Thr180) (Cell Signalling, 3681), KAP1 (Bethyl, A300‐274A) and phospho‐KAP1S824 (Cell signalling, 4127).

Pefani D.E., Tognoli M.L., Pirincci Ercan D., Gorgoulis V, & O'Neill E. (2018). MST2 kinase suppresses rDNA transcription in response to DNA damage by phosphorylating nucleolar histone H2B. The EMBO Journal, 37(15), e98760.

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Immunofluorescence Staining of Nucleolin

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Cells were cultured on coverslips, fixed with 4 % formaldehyde for 10 min, washed with PBS and afterwards blocked for 1 h in 1 % BSA/PBS-T at RT. The cells were then incubated with the primary antibody (Nucleolin 4E2, Abcam) over night (4 °C) or lectin (VVL-FITC, Vector) for 1 h at RT and washed with PBS. After incubation with the primary antibody, a fluorescent secondary antibody (Alexa Fluor 546 rabbit anti mouse IgG, Life Technologies) was added for 1 h at RT. After washing with PBS, the specimens were observed under a fluorescence microscope (BIOREVO BZ-9000; Keyence) and analyzed with BZ-9000 Generation II Analyzer software.

Hofmann B.T., Schlüter L., Lange P., Mercanoglu B., Ewald F., Fölster A., Picksak A.S., Harder S., El Gammal A.T., Grupp K., Güngör C., Drenckhan A., Schlüter H., Wagener C., Izbicki J.R., Jücker M., Bockhorn M, & Wolters-Eisfeld G. (2015). COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer. Molecular Cancer, 14, 109.

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