[2,2′-azino-bis-(3-ethylbenzo-thiazoline-6-sulfonic acid)] (ABTS free radical), D-galactose and ascorbic acid (VC) were received from Solarbio Science and Technology Co., Ltd. (Beijing, China). LC grade acetonitrile was supplied by Thermo Fisher Scientific Inc. (USA). Caffeic and rosmarinic acids standards were purchased from SIGMA-ALDRICH Co., Ltd. (USA). Ferulic acid was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China). 2,2′-diphenyl-1-picrylhydrazyl (DPPH free radical) was purchased from TCI Development Co., Ltd. (Shanghai, China). The biochemical assay kits used for determination of superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GSH-px) were received from Elabscience Biotechnology Co., Ltd. (Wuhan, China). All the chemicals used in the study were of analytical grade.
Gsh px
Manufactured by Elabscience
Sourced in United States, China
GSH-Px is a lab equipment product that measures the activity of the enzyme glutathione peroxidase (GSH-Px). GSH-Px is an antioxidant enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohol, respectively, using reduced glutathione as a substrate.
Automatically generated - may contain errors
Lab products found in correlation
Varioskan lux, by Thermo Fisher Scientific (2 mentions)
Ascorbic acid vc, by Solarbio (1 mentions)
Lc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Ferulic acid, by Yuanye Bio-Technology (1 mentions)
D galactose, by Solarbio (1 mentions)
A1r mp, by Nikon (1 mentions)
T sod, by Elabscience (1 mentions)
Hematoxylin and eosin h e, by Merck Group (1 mentions)
Tris hydrochloride tris hcl, by Merck Group (1 mentions)
6 protocols using gsh px
1
Antioxidant and Enzyme Assays Protocol
2
Quantifying Myocardial Oxidative Stress
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The LV tissues were homogenized and centrifuged (8000 rpm, 15 min, 4 °C). Following that, the supernatants were collected for oxidative stress tests. The malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) were assessed matched to the supplier’s instructions (Elabscience, Wuhan, China). The intact cardiac tissues stored at −80 °C were used to make frozen preparations. 2,7-Dichlorofluorescein diacetate (DCFH-DA) was applied for observing the cytosolic superoxide anion to assess the reactive oxygen species (ROS) production in the myocardium. To quantify the ROS level, DCFH fluorescence intensity was measured using a confocal microscope (Nikon, A1RMP+, Japan).
3
Glutathione Peroxidase Activity Assay
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Reduced glutathione in the sample was reacted with dinitrobenzoic acid (Elabscience, USA) to form a yellow complex, which was detected at 405 nm using Varioskan™ LUX (Thermo Fisher Scientific, USA). GSH at 1 μmol/L was consumed by 1 mg of GSH-Px (Elabscience, USA) at 37°C for 5 minutes, forming a coloring product that was detected at 412 nm using Varioskan™ LUX (Thermo Fisher Scientific, USA).
4
Antioxidant System Analysis in Brain
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Brain tissues and cells were collected and homogenized using ice-cold PBS. Supernatants were obtained for antioxidant system analysis. The levels of malondialdehyde (MDA) (Elabscience, Cat# E-BC-K027-M, E-BC-K028-M, China), glutathione peroxidase (GSH-Px) (Elabscience, Cat# E-BC-K096-M, China), glutathione (GSH) (Elabscience, Cat# E-BC-K030-M, China), catalase (CAT) (Elabscience, Cat# E-BC-K031-M, China), and total superoxide dismutase (T-SOD) (Elabscience, Cat# E-BC-K019-M, China) were analyzed using assay kits following the manufacturer's instructions.
5
GSH-Px Activity Quantification
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1 μmol/L of GSH was consumed by 1 mg of GSH-Px (Elabscience, USA) at 37°C for 5 minutes, forming a coloring product that was detected at 412 nm using Varioskan™ LUX (Thermo Fisher Scientific, USA).
6
Doxorubicin-Induced Cardiac Injury Model
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DOX was bought as Adrimisin® (50 mg/25 mL injectable solution) from Saba Pharmaceuticals (Istanbul, Turkey). CGA (Cas Number #327-97-9) and Tris hydrochloride (Tris–HCl; Cas Number #1185-53-1) were obtained from Merck (Darmstadt, Germany). Sevoflurane anesthesia (Sevorane liquid, 100%) was obtained from Abbott Laboratory (Istanbul, Turkey). CK-MB (Cat.No.:E-EL-R1327), LDH (Cat.No.:E-BC-K046-M), cTn-T (Cat.No.: E-EL-R0151), malondialdehyde (MDA; Cat.No.:E-EL-0060), GSH(Glutathione; Cat.No.:E-EL-0026), GSH-PX (Cat.No.:E-BC-K096-S), SOD (Cat.No.:E-BC-K019-S), and CAT (Cat.No.:E-BC-K031-S) were bought from Elabscience (Houston, TX, USA). Hematoxylin and eosin (H&E; Cat.No.:1051750500) was obtained from Merck (Darmstadt, Germany). 8-hydroxy-2′-deoxyguanosine 8-OhDG (Cat.No.:ab48508), 4-hydroxynonenal (4-HNE; Cat.No.: ab46545), and dityrosine (DT; Cat.No.: ab243067) primary antibodies were obtained from Abcam (Boston, MA, USA). Horseradish peroxidase (HRP) (Cat.No.: TP-125-HL) was bought from Thermo Fisher (Istanbul, Turkey).
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