Representative cDNA from single cells across three C1 runs and Smart-Seq2 (on plates) were assessed using High Sensitivity DNA chips for Bioanalyzer (5067-4626 and 5067-4627; Agilent Technologies). Single cell cDNA from SMARTer3 (link),28 (link),29 (link),31 (link),32 (link) and Smart-Seq2 C1 IFCs and Smart-seq2 (on plates) was tagmented and pooled to make libraries using Illumina Nextera XT DNA sample preparation kit (Illumina; FC-131-1096) with 96 dual barcoded indices (Illumina; FC-131-1002). The library clean-up and sample pooling was performed using AMPure XP beads (Agencourt Biosciences; A63880). All protocols are described in the Fluidigm protocol (100-5950), Fluidigm Script Hub and Smart-seq2 protocol40 (link). The STRT-Seq libraries were made and sequenced at Karolinska Institutet as previously described9 (link),20 (link). The Single cell libraries from SMARTer and Smart-Seq2 C1 IFCs and Smart-seq2 (on plates) was sequenced across 1 lane of HiSeq V4 (Illumina) using 75bp/125bp paired-end sequencing.
96 dual barcoded indices
Manufactured by Illumina
The 96 dual barcoded indices are a set of 96 unique dual index sequences designed for use with Illumina's sequencing platforms. These indices enable the multiplexing of samples during library preparation, allowing multiple samples to be combined and sequenced simultaneously.
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2 protocols using 96 dual barcoded indices
1
Single-cell cDNA profiling and library preparation
2
Single-cell cDNA profiling and library preparation
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Representative cDNA from single cells across three C1 runs and Smart-Seq2 (on plates) were assessed using High Sensitivity DNA chips for Bioanalyzer (5067-4626 and 5067-4627; Agilent Technologies). Single cell cDNA from SMARTer3 (link),28 (link),29 (link),31 (link),32 (link) and Smart-Seq2 C1 IFCs and Smart-seq2 (on plates) was tagmented and pooled to make libraries using Illumina Nextera XT DNA sample preparation kit (Illumina; FC-131-1096) with 96 dual barcoded indices (Illumina; FC-131-1002). The library clean-up and sample pooling was performed using AMPure XP beads (Agencourt Biosciences; A63880). All protocols are described in the Fluidigm protocol (100-5950), Fluidigm Script Hub and Smart-seq2 protocol40 (link). The STRT-Seq libraries were made and sequenced at Karolinska Institutet as previously described9 (link),20 (link). The Single cell libraries from SMARTer and Smart-Seq2 C1 IFCs and Smart-seq2 (on plates) was sequenced across 1 lane of HiSeq V4 (Illumina) using 75bp/125bp paired-end sequencing.
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