Wild-type (WT) or MUT HAGLR binding to miR-182-5p was subcloned into the pGL3 vector. BMSCs were co-transfected with miR-182-5p (RiboBio, Guangzhou, China) and 10 μg of pur-WT-HAGLR or pur-MUT-HAGLR. WT or MUT Hoxa10 and miR-182-5p were subcloned into a pGL3-based vector (Promega). miR-182-5p (RiboBio) was co-transfected with 10 μg pluco-WT-Hoxa10 or pluco-MUT-Hoxa10. After transfection of 48 h, a test of luciferase activity was done via the dual luciferase detection system (Promega Corporation, Fitchburg, WI, USA) [38 (link)].
Mir 182 5p
Manufactured by RiboBio
Sourced in China
MiR-182-5p is a microRNA (miRNA) product offered by RiboBio. miRNAs are small, non-coding RNA molecules that play a role in regulating gene expression. This specific miRNA, MiR-182-5p, is a sequence-verified RNA oligonucleotide.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using mir 182 5p
1
Luciferase Assay for miR-182-5p Targets
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