MALDI-TOF mass spectrometry was carried out on an ultrafleXtreme MALDI TOF/TOF instrument (BrukerDaltonics), with the use of Peptide Calibration Standard (BrukerDaltonics) as external calibration and Norharmane (9H-Pyrido[3,4-b]indole; Sigma) as a matrix (10 mg/ml, chloroform:methanol, 2:1, v/v). GSLs were dissolved in chloroform/methanol (2:1, v/v). Spectra were scanned in the range of m/z 700–2000 in the reflectron-positive mode.
Norharmane 9h pyrido 3 4 b indole
Manufactured by Merck Group
Sourced in United States
Norharmane (9H-Pyrido[3,4-b]indole) is a heterocyclic aromatic compound. It is a solid crystalline substance.
Automatically generated - may contain errors
3 protocols using norharmane 9h pyrido 3 4 b indole
1
MALDI-TOF MS Analysis of Glycosphingolipids
2
MALDI-TOF Analysis of Glycosphingolipids
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MALDI–TOF (matrix-assisted laser desorption/ionization–time-of-flight) mass spectrometry of glycosphingolipids was carried out on ultrafleXtreme MALDI–TOF/TOF instrument (Bruker Daltonics, Bremen, Germany). Samples were dissolved in chloroform/methanol (2:1, v/v). Norharmane (9H-pyrido[3,4-b]indole (Sigma-Aldrich, St. Louis, MO, USA) was used as a matrix (10 mg/mL, chloroform/methanol, 2:1, v/v). Spectra were scanned in the range of m/z 700–3500 in the reflectron-positive mode. External calibration was applied using the peptide calibration standard (Bruker Daltonics, Bremen, Germany).
3
MALDI-TOF Mass Spectrometry Protocol
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MALDI-TOF mass spectrometry was carried out on a MALDI TOF/TOF ultrafleXtreme™ instrument (BrukerDaltonics, Bremen, Germany). Samples were dissolved in chloroform/methanol (2:1, v/v). Norharmane (9H-Pyrido [3,4-b] indole, Sigma-Aldrich, St.
Louis, MO) was used as a matrix (10 mg/ml, chloroform/methanol, 2:1, v/v). Spectra were scanned in the range of m/z 700-1600 in the reflectron-positive mode. External calibration was applied using the Peptide Calibration Standard II (BrukerDaltonics, Bremen, Germany).
Louis, MO) was used as a matrix (10 mg/ml, chloroform/methanol, 2:1, v/v). Spectra were scanned in the range of m/z 700-1600 in the reflectron-positive mode. External calibration was applied using the Peptide Calibration Standard II (BrukerDaltonics, Bremen, Germany).
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