Cell-free supernatants were harvested and analyzed for levels of TNFα, IL-6, and IL-12p40 (BD Biosciences, Heidelberg, Germany) by ELISA according to the manufacturer's instructions. For evaluation of systemic cytokine levels in mice at indicated time points, whole mouse blood was transferred to microtubes with serum gel containing a clotting activator (Sarstedt, Nümbrecht, Germany), left undisturbed for 30 min and then centrifuged for 10 min at 10,000 g to harvest the serum. Serum cytokine profile was determined using either ELISA, the LEGENDplex mouse inflammation panel (Biolegend, San Diego, CA), or a Luminex-based multiplex assay (Bio-Rad, Hercules, USA) according to the manufacturer's instructions.
Luminex based multiplex assay
Manufactured by Bio-Rad
Sourced in United States
The Luminex-based multiplex assay is a powerful tool for simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded magnetic beads coated with specific capture antibodies to capture and measure target proteins or molecules. This technology enables efficient and high-throughput analysis, providing researchers with a versatile platform for various applications.
Automatically generated - may contain errors
Lab products found in correlation
Il 12p40, by BD (1 mentions)
Legendplex mouse inflammation panel, by BioLegend (1 mentions)
Interleukin 6 (il 6), by BD (1 mentions)
Clotting activator, by Sarstedt (1 mentions)
Tnf α, by BD (1 mentions)
Capture antibody, by R&D Systems (1 mentions)
Biotinylated detection antibody, by R&D Systems (1 mentions)
3 protocols using luminex based multiplex assay
1
Cytokine Profiling in Cell Culture and Mice
2
Multiplex Cytokine Profiling in Liver Transplantation
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A total of 50 chemokines and growth factors in sera of patients who underwent liver transplantation were quantified by Luminex-based multiplex assay according to the manufacturer’s instruction (Bio-Rad, USA). The detection range of all cytokines was between 0.1 pg/mL and 40 µg/mL. Only data points where ‘pre-OLT’ and ‘post-OLT’ measurements for any given patient and mediator were technically successful and within the dynamic range of the assay were included in the analysis.
3
Simultaneous Evaluation of LDL, HDL, and Serum Proteins
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On a 96-wells plate, LDL, HDL and whole serum were measured simultaneously. Protein concentrations in EV fractions and serum were measured in a well-established protein panel consisting of: CD14, cystatin C, serpin C1 and serpin G1 [20 (link)]. Capture antibody, biotinylated detection antibody and antigen of all four proteins were purchased from R&D systems. Proteins were quantitatively analyzed by Luminex-based multiplex assay (Bio-Rad, Austin, USA). All EV protein levels were corrected for total amount of protein.
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