Log In Sign up
The largest database of trusted experimental protocols

Ab246887

Manufactured by Abcam
Visit Supplier

Ab246887 is a primary antibody designed for the detection of a specific target protein. It is a high-quality reagent suitable for use in various immunoassay applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab26350, by Abcam (2 mentions) Ab150114, by Abcam (2 mentions) Fl 1321 2, by Vector Laboratories (2 mentions) Ab150077, by Abcam (2 mentions) Ab15580, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Fv3000, by Olympus (1 mentions) Ab1423, by Abcam (1 mentions) Ab9566, by Abcam (1 mentions)

2 protocols using ab246887

1

Immunofluorescence Analysis of Kidney Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence analysis was performed on kidney sections (2-μm thickness) and HK-2 cells. Slides were incubated with antibodies against anti-γH2AX (ab26350, Abcam), anti-CDK12 (ab246887, Abcam), anti-Ki-67 (ab15580, Abcam), anti-PCNA (ab29, Abcam), and LTL (FL-1321-2, Vector Laboratories) and incubated with secondary antibodies (ab150114 and ab150077, Abcam). Nuclei were stained with DAPI (Sigma-Aldrich) according to the manufacturer's instructions. Under a confocal microscope (FV3000, Olympus), 10 fields of view were randomly assigned, and the number of CDK12-positive cells was counted in a blinded manner.
Zhang Y.L., Tang T.T., Ni W.J., Li Z.T., Jiang L.Y., Wang Y., Zhou X., Cao J.Y., Yin Q., Jiang W., Zhao Y.J., Gan W.H., Zhang A.Q., Li Z.L., Wen Y., Lv L.L., Liu B.C, & Wang B. (2024). Tubule-specific cyclin-dependent kinase 12 knockdown potentiates kidney injury through transcriptional elongation defects. International Journal of Biological Sciences, 20(5), 1669-1687.
+ Open protocol
+ Expand
2

Immunofluorescence Analysis of Renal Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunofluorescence analysis was performed on 4 μm thick renal tissue sections and HK-2 cells. They were performed with anti-TRF1 (ab1423, Abcam), anti-γH2AX (ab26350), anti-CDK12 (ab246887, Abcam) , AQP1 (ab 9566, Abcam) , LTL (FL-1321-2, Vector Laboratories) and incubated with secondary antibodies (ab150114 and ab150077, Abcam). Under the confocal microscope, 10 fields of view were randomly assigned, and the number of positive tubules and positive cells was counted in a blind manner.
Yin Q., Zhao Y.J., Ni W.J., Tang T.T., Wang Y., Cao J.Y., Yin D., Wen Y., Li Z.L., Zhang Y.L., Jiang W., Zhang Y., Lu X.Y., Zhang A.Q., Gan W.H., Lv L.L., Liu B.C, & Wang B. (2022). MiR-155 deficiency protects renal tubular epithelial cells from telomeric and genomic DNA damage in cisplatin-induced acute kidney injury. Theranostics, 12(10), 4753-4766.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!