Spss software v 18

Follow-up data available as of Dec 31, 2017, were analyzed. The locoregional control (LRC) was defined as freedom from clinical or radiographic evidence of locoregional failure (LRF) within the ipsilateral CW and/or regional draining lymphatics after treatment with radiation for initial ILRR. The distant metastasis-free survival (DMFS) was measured from the date of diagnosis of ILRR to the date of DM. The disease-free survival (DFS) was measured from the date of diagnosis of ILRR to the date of second ipsilateral LRR, DM, or death attributable to breast cancer and/or second primary nonbreast cancer. The OS was defined as the time from the diagnosis of ILRR to death attributable to breast cancer, cause other than breast cancer, or unknown cause at the last follow-up date. Statistics were performed using SPSS software (v18.0). The probabilities of LRC, DMFS, DFS, and OS were calculated using the Kaplan-Meier product-limit method, and compared between groups using the log-rank test. The influence of primary tumor characteristics, recurrence patterns, and treatment-related factors after ILRR on LRC, DFS, and OS were tested by univariate and multivariate analysis using forward step-wise Cox regression method. All p values were two-sided and if less than 0.05 were deemed significant.

RT-qPCR data were present as means ± standard error of the mean (SEM), other quantitative data were shown as means ± standard deviation (SD). SPSS software v 18.0 (USA) was used for statistical analysis with the tank as the statistical unit. Two-way analysis of variance (ANOVA) followed by Dunnett’s test was employed for all data analysis, except the Chi-square test for the development stage distribution of spontaneous metamorphosis assay among TBBPA treatments.

The test consists of a transparent plastic box placed in a table with the four edges emerging about 20 cm above the top of the table and a rod running along the middle, separating the box into two sections: one has a checker paper placed on the top surface of the box, whereas the other has the same checker paper placed on the bottom surface of the box. The mouse was placed on the rod and should choose within 5 min to walk on the safe side, where the checker paper is on the top surface of the box or the cliff zone (“fake cliff”) where the checker paper is on the bottom surface of the box. Each mouse is tested for 10 trials with the apparatus turned 180° after five trials. Results are expressed in percentages of chosen zones for each mouse.
Statistics were done using SPSS Software, v18.0. One or Two-Way analysis of variance (ANOVA) followed by Bonferroni post-hoc comparisons was used to compare the mean differences with multiple factors, i.e., between groups (genotypes), and/or intervals (days), as appropriate. Unpaired Student t-test was used when only two groups were compared, unless specified otherwise. Equality of variances was assessed by Levene's Test. Statistical significance was considered only when p < 0.05.